{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:12:15Z","timestamp":1699834335079},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":12909,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1971,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A recently advanced generalization is that a sialomucin coat surrounding tumour cells protects them from immunological surveillance. We have therefore studied the effects of neuraminidase (sialidase) on the immunolysis of P815 mastocytoma cells by sensitized spleen cells, <jats:italic>in vitro<\/jats:italic>, to test this hypothesis. In a very sensitive system in which immunolysis was quantitated by measurements of the release of <jats:sup>51<\/jats:sup>Cr from labelled P815 cells, neuraminidase treatment of the target cells <jats:italic>did not<\/jats:italic> demonstrably make them more susceptible to attack by spleen cells. Killing proceeded in spite of a substantial contribution of sialic acid moieties to the net negative surface charge of the untreated P815 cells, and was not enhanced by enzymatic removal of substantial amounts of their bound surface sialic acids, as determined by cell electrophoresis. Thus, the generalization cannot be substantiated by our experiments. An interesting positive finding was that neuraminidase treatment of the killer, spleen cell population, enhances the <jats:sup>51<\/jats:sup>Cr\u2010release from their targets.<\/jats:p>","DOI":"10.1002\/ijc.2910070202","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T13:45:34Z","timestamp":1171892734000},"page":"187-197","source":"Crossref","is-referenced-by-count":15,"title":["Some effects of neuraminidase on the <i>in vitro<\/i> interactions between spleen and mastocytoma (P815) cells"],"prefix":"10.1002","volume":"7","author":[{"given":"L.","family":"Weiss","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. L.","family":"Cudney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"247","volume-title":"Proc. 7th Canadian Cancer Res. Conf.","author":"Ambrose E. J.","year":"1967"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(69)90205-5"},{"key":"e_1_2_1_4_1","first-page":"181","article-title":"Quantitative assay of the lytic action of immune lymphoid cells on 51Cr labelled allogenic target cells in vitro: Inhibition by isoantibody and by drugs","volume":"14","author":"Brunner K. T.","year":"1968","journal-title":"Immunology"},{"key":"e_1_2_1_5_1","first-page":"637","article-title":"Glycoprotein coat of the TA3 cell. I. Removal of carbohydrate and protein material from viable cells","volume":"45","author":"Codington J. F.","year":"1970","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1968.tb00964.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(62)90309-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(67)92183-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1968.70"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1968.99"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1969.21"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030620105"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(62)90151-9"},{"key":"e_1_2_1_14_1","first-page":"79","volume-title":"Cellular recognition","author":"Gesner B. M.","year":"1969"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/190269a0"},{"key":"e_1_2_1_16_1","unstructured":"Hauschka T. S. Weiss L.Holdridge B. A. Zumpft M. Cudney T. L. andPlaninsek J. A. Karyotypic and surface features of murine TA3 carcinoma cells during immunoselection in mice and rats.J. nat. Cancer Inst. In Press (1971)."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(70)90446-5"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.49.4.717"},{"key":"e_1_2_1_19_1","unstructured":"Mayhew E. andBennett M. In preparation (1971)."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(68)90462-X"},{"key":"e_1_2_1_21_1","first-page":"405","article-title":"Suspension cell culture and in vivo and in vitro constitution of mouse leukaemia L1210","volume":"36","author":"Moore G. E.","year":"1966","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(66)90459-9"},{"key":"e_1_2_1_23_1","volume-title":"Int. Symp. Immunopathology","author":"Perlmann P.","year":"1967"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/1811586a0"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196709000-00010"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196709000-00005"},{"key":"e_1_2_1_27_1","first-page":"268","volume-title":"Cell membrane properties in neoplasia","author":"Vassar P. S.","year":"1967"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1016\/S0021-9258(18)69851-5","article-title":"The thiobarbituric assay of sialic acid","volume":"234","author":"Warren L.","year":"1959","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.26.3.735"},{"key":"e_1_2_1_30_1","volume-title":"The cell periphery, metastasis and other contact phenomena","author":"Weiss L.","year":"1967"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61634-4"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","unstructured":"Weiss L. Cell contact phenomena. in vitro 5 48\u201378(1970).","DOI":"10.1007\/BF02618374"},{"key":"e_1_2_1_33_1","volume-title":"The chemistry of bio\u2010surfaces","author":"Weiss L.","year":"1971"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910030613"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910060214"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(66)80110-6"},{"key":"e_1_2_1_37_1","first-page":"1304","article-title":"The effect of neuraminidase on the phagocytic process in human monocytes","volume":"15","author":"Weiss L.","year":"1966","journal-title":"Lab. Invest."},{"key":"e_1_2_1_38_1","first-page":"90","article-title":"The electrokinetic surfaces of human cells of lymphoid origin and their ribonuclease susceptibility","volume":"30","author":"Weiss L.","year":"1970","journal-title":"Cancer Res."},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196505000-00011"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910070202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910070202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T08:11:13Z","timestamp":1699776673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910070202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,3,15]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1971,3,15]]}},"alternative-id":["10.1002\/ijc.2910070202"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910070202","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,3,15]]}}}