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The adsorption of the virus to the outer cell membrane was assessed by counting the number of direct membrane fluorescence\u2010positive cells immediately after infection and by the direct radioimmune membrane labelling method. Reference reagents were derived for both tests from the same serum (\u201cAgnes\u201d), containing antibodies against EB\u2010viral envelope and capsid antigens. The intracellular course of infection was followed by counting the number of cells that responded with the development of early antigen (EA) 48 h after infection. The 11 lymphoblastoid lines that produced no EBV\u2010determined membrane and early antigens adsorbed the virus, although there were quantitative differences between them. EA\u2010positive cells appeared in significant numbers in only seven of them, however. Four lines remained EA negative in spite of a relatively good adsorbing capacity. The IgE\u2010producing myeloma line showed neither virus adsorption nor EA development.<\/jats:p><jats:p>Eighteen lymphoblastoid lines were \u201cproducers\u201d, or \u201cabortive producers\u201d, <jats:italic>i.e.<\/jats:italic>a small proportion of the cells continuously generated two or three of the immunofluorescence\u2010detectable viral antigens, MA, EA and VCA. Nine lines failed to adsorb significant virus quantities and showed no certain increase of EA\u2010positive cells. The resistance of these lines to superinfection is probably determined at the level of viral receptors. Five lines showed a relatively good virus adsorption, but this was not followed by any significant increase in the number of EA\u2010positive cells. Four lines showed good adsorption and also responded with a significant increase in the number of EA\u2010positive cells.<\/jats:p><jats:p>The same responses can thus be found to EBV\u2010superinfection in producer and non\u2010producer lines, but the producer lines show a strong preponderance of superinfection\u2010resistant lines with an adsorption block at the receptor level.<\/jats:p>","DOI":"10.1002\/ijc.2910100108","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T13:55:22Z","timestamp":1171893322000},"page":"44-57","source":"Crossref","is-referenced-by-count":235,"title":["Sensitivity of Epstein\u2010Barr virus (EBV) producer and non\u2010producer human lymphoblastoid cell lines to superinfection with EB\u2010virus"],"prefix":"10.1002","volume":"10","author":[{"given":"George","family":"Klein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laszlo","family":"Dombos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balwant","family":"Gothoskar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"469","article-title":"Initiation and establishment of lymphoid cell lines from the blood of healthy persons","volume":"47","author":"Chang S. 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