{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:35:19Z","timestamp":1779345319033,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":11295,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1975,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Studies were performed to characterize the effector cells responsible for natural cytotoxicity of mouse lymphoid cells against a variety of syngeneic and allogeneic tumor lines. Since spleen cells from normal nude mice were found to be highly cytotoxic, they were used for most of these experiments. Only a small proportion of the reactivity was affected by treatment with anti\u2010\u03b8 serum plus complement. Macrophages did not appear to be responsible for the reactivity, since treatment with carbonyl iron\/magnet or carrageenan did not affect the levels of cytotoxicity. The effector cells were non\u2010adherent, since passage over nylon columns resulted in a considerable increase in activity. The active cells did not have receptors for immunoglobulin or complement, since removal of cells with these receptors by columns or monolayers containing sheep erythrocyteantibody (EA) complexes or EA\u2010complement complexes did not remove activity. Antibody\u2010dependent cell\u2010mediated cytotoxicity appeared to be ruled out as the mechanism for natural cytotoxicity, since aggregated gamma globulin and a potent anti\u2010immunoglobulin reagent did not inhibit reactivity, and since no role for humoral factors could be demonstrated. The natural effector cell was found to be quite labile at 37\u00b0 C, losing much of its activity after 4 h. Since no surface markers could be detected on the effector cells, and the mechanism for cytotoxicity appeared distinct from others previously described, it is proposed that the natural cytotoxicity against mouse tumor cells is mediated by a unique subpopulation of lymphoid cells, which are tentatively designated N\u2010cells.<\/jats:p>","DOI":"10.1002\/ijc.2910160205","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T15:47:57Z","timestamp":1171900077000},"page":"230-239","source":"Crossref","is-referenced-by-count":664,"title":["Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells"],"prefix":"10.1002","volume":"16","author":[{"given":"Ronald B.","family":"Herberman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myrthel E.","family":"Nunn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard T.","family":"Holden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Lavrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"761","article-title":"Quantitative in vitro, assay of cytotoxic cellular immunity","volume":"45","author":"Canty T. G.","year":"1970","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60308-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio232076a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.135.4.890"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio242111a0"},{"key":"e_1_2_1_7_1","first-page":"99","article-title":"Spontaneously arising cytotoxicity to the P\u2010815\u2010Y mastocytoma in NZB mice","volume":"16","author":"Greenberg A. H.","year":"1974","journal-title":"Clin. exp. Immunol."},{"key":"e_1_2_1_8_1","first-page":"251","article-title":"Characterization of the antibody\u2010dependent cytotoxic\u2010cell. A non\u2010phagocytic monocyte?","volume":"15","author":"Greenberg A. H.","year":"1973","journal-title":"Clin. exp. Immunol."},{"key":"e_1_2_1_9_1","first-page":"2886","article-title":"Enhancement of normal cytotoxicity by sera with high antibody titers against H\u20102 or virus\u2010associated antigens","volume":"33","author":"Harada M.","year":"1973","journal-title":"Cancer Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820137209022895"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910160204"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/51.5.1509"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio235048a0"},{"key":"e_1_2_1_14_1","first-page":"163","article-title":"Human lymphocyte subpopulations: classification according to surface markers and\/or functional characteristics","volume":"16","author":"Jondal M.","year":"1973","journal-title":"Transplant Rev."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830031011"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.4049\/jimmunol.112.3.1231","article-title":"Cellular immunoabsorbents. A simplified technique for separation of lymphoid\u2010cell populations","volume":"112","author":"Kedar E.","year":"1974","journal-title":"J. Immunol."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830050209"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.6.1473"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","unstructured":"Kirchner H. Holden H. T. andHerberman R. B. Inhibition of in vitro growth of lymphoma cells by macrophages from tumor\u2010bearing mice.J. nat. Cancer Inst. in press (1975a).","DOI":"10.1093\/jnci\/55.4.971"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.4049\/jimmunol.114.1_Part_1.206","article-title":"Inhibition of proliferation of lymphoma cells and T\u2010lymphocytes by suppressor cells from spleens of tumorbearing mice","volume":"114","author":"Kirchner H.","year":"1975","journal-title":"J. Immunol."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.137.6.1472"},{"key":"e_1_2_1_22_1","first-page":"675","article-title":"Cytotoxicity of human lymphocytes induced by rabbit antibodies to chicken erythrocytes","volume":"25","author":"Larsson A.","year":"1973","journal-title":"Immunology"},{"key":"e_1_2_1_23_1","first-page":"459","article-title":"Effector activating determinants on IgG. The distribution and factors influencing the display of complement, neutrophil and cytotoxic B\u2010cell determinants on human IgG sub\u2010classes","volume":"25","author":"MacLennan I. C. M.","year":"1973","journal-title":"Immunology"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.4049\/jimmunol.112.4.1477","article-title":"Comparative in vitro studies on effector\u2010cell diversity in the cellular immune response to murine response to murine sarcoma virus (MSV)\u2010induced tumors in mice","volume":"112","author":"Plata F.","year":"1974","journal-title":"J. Immunol."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910090209"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/246350a0"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.120.3.413"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.4049\/jimmunol.112.6.2219","article-title":"Cytotoxic immune cells with specificity for defined soluble antigens. V. Interaction of antibody with the cytotoxic effector cells in immune or nonimmune mouse spleen cells","volume":"112","author":"Schirrmacher V.","year":"1974","journal-title":"J. Immunol."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910140313"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.4049\/jimmunol.110.4.1027","article-title":"Antibody\u2010dependent lymphoid\u2010cellmediated cytotoxicity: role of lymphocytes bearing a receptor for complement","volume":"110","author":"van Boxel J. A.","year":"1973","journal-title":"J. Immunol."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4018.194"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(74)90178-6"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1973.tb02026.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000231208"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910160205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910160205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:12:41Z","timestamp":1699765961000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910160205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,8,15]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,8,15]]}},"alternative-id":["10.1002\/ijc.2910160205"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910160205","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,8,15]]}}}