{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:15:04Z","timestamp":1760865304134},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":8554,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1983,2,15]]},"abstract":"Abstract<\/jats:title>Human plasma cryoprecipitates (cryos) of tumor patients have been shown to contain transformation\u2010enhancing factors (TEF)4<\/jats:sup>, able to promote morphological cell transformation in vitro.<\/jats:italic> A similar activity is associated with proteolytic fibronectin fragments retaining the gelatin\u2010binding site. We have now studied whether similarities exist between TEF found in cryos and the fibronectin fragments. Of 14 TEF\u2010positive tumor cryos tested, 13 (including carcinomas, leukemias, lymphomas and myelomas) retained their activity in the gelatin\u2010binding fraction, and in one the activity was not recovered. In one case of myeloma, TEF activity was detected only in the gelatin\u2010binding fraction and not in the starting cryo. In contrast, none of the TEF\u2010negative control samples tested, obtained from healthy donors and patients affected by different non\u2010malignant diseases, showed any TEF activity in the gelatin\u2010binding or \u2010nonbinding fractions. The TEF\u2010positive gelatin\u2010binding fractions of tumor cryos were active even at the concentration of \u223c 50 ng\/ ml. The presence of fibronectin in human plasma cryos was detected using immunoblotting. In the gelatin\u2010binding fractions, fibronectin antigenicity was seen in the position of intact fibronectin subunits (Mr<\/jats:sub> = 220,000) and different fragments (Mr<\/jats:sub> = 50,000\u2010200,000). while the gelatin\u2010binding fractions contained a single antigenic polypeptide (Mr<\/jats:sub> \u223c94,000). The TEF activity of cryo, as well as that of plasminolytic digest of purified plasma fibronectin, could be inhibited by fibronectin antibodies. The data suggest that the' TEF activity present in tumor patient plasma cryoprecipitates is related to gelatin\u2010binding fragments of fibronectin.<\/jats:p>","DOI":"10.1002\/ijc.2910310205","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:10:18Z","timestamp":1171912218000},"page":"157-162","source":"Crossref","is-referenced-by-count":23,"title":["Transformation\u2010enhancing activity in plasma of tumor patients: Relationship with fibronectin fragments"],"prefix":"10.1002","volume":"31","author":[{"given":"Giuseppina","family":"De Petro","sequence":"first","affiliation":[]},{"given":"Sergio","family":"Barlati","sequence":"additional","affiliation":[]},{"given":"Tapio","family":"Vartio","sequence":"additional","affiliation":[]},{"given":"Antti","family":"Vaheri","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"303","volume-title":"Protides of the biological fluids","author":"Barlati S.","year":"1982"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149840"},{"key":"e_1_2_1_4_1","first-page":"269","volume-title":"Control mechanisms in animal cells","author":"Barlati S.","year":"1980"},{"key":"e_1_2_1_5_1","first-page":"331","volume-title":"Avian RNA tumor viruses","author":"Barlati S.","year":"1978"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149450"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(72)90280-2"},{"key":"e_1_2_1_8_1","first-page":"407","article-title":"Statistical accuracy to be expected from cell colony assays: with special reference to the spleen colony assay","volume":"7","author":"Blackett N. 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A method for evaluating the statistical accuracy ofin vitrotitration.Microbiologica(in press) (1982)."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910250607"},{"key":"e_1_2_1_19_1","unstructured":"Mignatti P. Barlati S. Poulillart P. Zucker J. M. andVigier P. A plasma biological activity associated with different types of malignancies.Proceedings of the VIth Meeting of the European Association for Cancer Research p.27 Budapest (abstract) (1981)."},{"key":"e_1_2_1_20_1","first-page":"111","article-title":"Fibronectin","volume":"5","author":"Mosher D. F.","year":"1980","journal-title":"Prog. Hemost. Tromb."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0174-173X(80)80011-2"},{"key":"e_1_2_1_22_1","first-page":"901","article-title":"Serum levels of fibronectin and a fibronectin\u2010like DNA\u2010binding protein in patients with various diseases","volume":"65","author":"Todd H. D.","year":"1980","journal-title":"J. nat. 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