{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:36:33Z","timestamp":1770503793161,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":7822,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1985,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>NIH 3T3 cells were transfected with DNA derived from human bladder carcinoma, colon carcinoma and HL60 promyelocytic leukemia cells. The transfectants were examined for the presence of human oncogenes in relation to tumorigenic potential and composition of surface\u2010located fucosyl glycopeptides by gel filtration, Concanavalin\u2010A\u2010binding and high\u2010performance liquid chromatography. All transfectants, harboring 3 different human cellular <jats:italic>ras<\/jats:italic> genes, appeared to be tumorigenic in nude mice and displayed characteristically altered glycopeptides. The surface glycopeptides were consistently changed to higher apparent molecular weight due to enrichment in higher\u2010branched sialic\u2010acid\u2010containing glycopeptides. Similar alterations have been found previously in virally\u2010 and chemically\u2010transformed cells <jats:italic>in vitro<\/jats:italic> and tumors raised <jats:italic>in vivo<\/jats:italic>, and were designated as cancer\u2010related or tumor\u2010associated glycopeptides. Revertants derived from HL60\u2010DNA\u2010induced transfectants, which had lost the transfected human N\u2010<jats:italic>ras<\/jats:italic> oncogene, simultaneously lost their tumorigenic potential and expression of cancer\u2010related membrane glycopeptides. In addition, spontaneous transformants, exhibiting morphology and growth patterns indistinguishable from those of tumor\u2010DNA\u2010induced transfectants, neither contained transferred human DNA sequences nor expressed cancer\u2010related glycopeptides. Nevertheless these cells were capable, after prolonged latency periods, of inducing tumors in nude mice. Cells derived from such tumors constantly displayed cancer\u2010related glycopeptides on their surface, suggesting selection of tumorigenic cells from spontaneous transformants during passage in nude mice. In one of these tumors at least, an endogenous mouse <jats:italic>ras<\/jats:italic>\u2010gene appeared to be activated. The results indicate a close correlation between the presence of activated <jats:italic>ras<\/jats:italic>\u2010oncogenes in the genome of the transfected cells, the tumorigenic potential of these cells and the expression of surface\u2010located cancer\u2010related glycopeptides. The data suggest that functions provided by human <jats:italic>ras<\/jats:italic>\u2010oncogenes contribute to the alteration of membrane glycopeptides on tumor cells.<\/jats:p>","DOI":"10.1002\/ijc.2910350211","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:17:37Z","timestamp":1171916257000},"page":"207-213","source":"Crossref","is-referenced-by-count":61,"title":["Transfection by human oncogenes: Concomitant induction of tumorigenicity and tumor\u2010associated membrane alterations"],"prefix":"10.1002","volume":"35","author":[{"given":"J. G.","family":"Collard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. P.","family":"van Beek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. W. G.","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"Schijven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910110327"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/270347a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6285471"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(82)90392-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.11.3637"},{"key":"e_1_2_1_7_1","first-page":"12","volume-title":"Proceedings of the Sixth International Symposium on Glycoconjugates, Tokyo, Japan","author":"Dorland L.","year":"1981"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/292506a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00579a015"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1128\/jvi.13.5.967-974.1974","article-title":"Surface membrane glycopeptides which coincide with virus transformation and tumorigenesis","volume":"13","author":"Glick M. C.","year":"1974","journal-title":"J. Virol."},{"key":"e_1_2_1_11_1","first-page":"2189","article-title":"Glycopeptide changes after transfection of NIH 3T3 with human tumor DNA. (Abstract)","volume":"42","author":"Glick M. C.","year":"1983","journal-title":"Fed. Proc."},{"key":"e_1_2_1_12_1","first-page":"501","article-title":"Surface properties of normal transformed and temperature\u2010Sensitive rat liver epithelial cells","volume":"66","author":"Goldstein N. J.","year":"1981","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90341-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/303396a0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90119-2"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6356358"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/302079a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90017-X"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/302839a0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/259580a0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90388-3"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/300539a0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/300149a0"},{"key":"e_1_2_1_24_1","first-page":"4967","article-title":"Correlation of surface fucopeptide patterns with tumorigenicity of mouse mammary epithelial cells","volume":"41","author":"Richards C. S.","year":"1981","journal-title":"Cancer Res."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/284372a0"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00579a016"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/298343a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90100-3"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(80)90022-0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90331-7"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(77)95004-2"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910180411"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/300143a0"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)90765-2"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90390-2"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/300762a0"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1977.172"},{"key":"e_1_2_1_39_1","first-page":"2913","article-title":"Increased sialic acid density in surface glycoproteins of tranformed and malignant cells\u2010A general phenomenon?","volume":"33","author":"Van Beek W. P.","year":"1973","journal-title":"Cancer Res."},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/253457a0"},{"key":"e_1_2_1_41_1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1182\/blood.V63.1.170.170","article-title":"Transient versus permanent expression of cancer\u2010Related glycopeptides on normal versus leukemic myeloid cells coinciding with marrow egress","volume":"63","author":"Van Beek W. P.","year":"1984","journal-title":"Blood"},{"key":"e_1_2_1_42_1","first-page":"5222","article-title":"Continuous expression of malignancy related fucosyl glycopeptides on the surface of human promyelocytic leukemia cells (HL\u201060) following terminal differentiation in vitro","volume":"42","author":"Van Beek W. P.","year":"1982","journal-title":"Cancer Res."},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00632a016"},{"key":"e_1_2_1_44_1","first-page":"97","article-title":"Glycopeptide changes and malignant transformation: a possible role for carbohydrate in malignant behavior","volume":"516","author":"Warren L.","year":"1978","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.7.1838"},{"key":"e_1_2_1_46_1","first-page":"2186","article-title":"The surface glycoproteins of a mouse melanoma growing in culture and as a solid tumor in vivo","volume":"35","author":"Warren L.","year":"1975","journal-title":"Cancer Res."},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.4.1087"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90093-X"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90091-4"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910350211","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910350211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T17:30:38Z","timestamp":1697823038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910350211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,2,15]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,2,15]]}},"alternative-id":["10.1002\/ijc.2910350211"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910350211","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,2,15]]}}}