{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:53:37Z","timestamp":1764996817203},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":7034,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1987,4,15]]},"abstract":"Abstract<\/jats:title>Peripheral or tonsil lymphocyte populations of EBV\u2010 seropositive donors give rise to EBV\u2010 carrying LCLs upon In vitro<\/jats:italic> explanation. Such lines can arise either by a 2\u2010step mechanism, namely release of virus from some of the explanted cells followed by infection of previously uninfected B cells, or by direct outgrowth of virus\u2010harboring B cells (Rickinson et al.<\/jats:italic>, 1974; Dalens et al.<\/jats:italic>, 1975; Hinuma and Katsuki 1978; Katsuki et al.<\/jats:italic>, 1979). We observed that cells responsible for both the 2\u2010step mechanism and for direct outgrowth are found in the purified B\u2010cell compartment. Virus release was more frequent than direct outgrowth. The majority of virus\u2010releasing cells were found in the low\u2010density fraction that contains large, activated B blasts. Cells that were capable of spontaneous outgrowth in the presence of the viral inhibitor PFA and of virus\u2010neutralizing antibody gave rise to cell lines that carried the sex chromosome marker of the original donor, rather than that of admixed cord blood lymphocyte of the opposite sex. Such cells were found in both the low\u2010 and the highdensity fractions. The majority of the EBV\u2010carrying B cells in vivo<\/jats:italic> are thus low\u2010density blasts. Rare small B cells of high density harboring EBV were capable of spontaneous outgrowth. This may be indicative of a host control mechanism that is removed upon cultivation in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910390411","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:43:25Z","timestamp":1171917805000},"page":"472-476","source":"Crossref","is-referenced-by-count":74,"title":["Characterization of ebv\u2010carrying b\u2010cell populations in healthy seropositive individuals with regard to density, release of transforming virus and spontaneous outgrowth"],"prefix":"10.1002","volume":"39","author":[{"given":"Nongnit","family":"Lewin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"\u00c5man","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Masucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"\u00d6berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Strander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Henle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertrude","family":"Henle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.159.1.208"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.4049\/jimmunol.135.4.2362","article-title":"Surface marker characterization of EBV target cells in normal blood and tonsil lymphocyte populations","volume":"135","author":"\u00c5man P.","year":"1985","journal-title":"J. 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A human cancer model for the study of the multiple development of cancer: Proposal for a new scenario","volume":"7","author":"Lenoir G.","year":"1987","journal-title":"Advanc. viral Oncol."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910220604"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910060103"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910080311"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910080312"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(82)90074-2"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/226078a0"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910110302"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/258236a0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910190606"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910140603"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(84)90032-7"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.161.5.1097"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.5.763"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(81)90220-3"},{"key":"e_1_2_1_30_1","article-title":"The Epstein\u2010Barr virus and the immune system","volume":"7","author":"Tosato G.","year":"1987","journal-title":"Advanc. viral Oncol."}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910390411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910390411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T23:00:50Z","timestamp":1697929250000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910390411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4,15]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4,15]]}},"alternative-id":["10.1002\/ijc.2910390411"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910390411","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4,15]]}}}