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Rabbits seroconverted to HTLV\u2010I, as determined by indirect immunofluorescence, by 3 weeks after inoculation and remained persistently seropositive during a 22\u2010month period of observation. Seroconversion did not occur in salineinoculated controls. Using Western immunoblotting and radio\u2010immunoprecipitation, persistent seroconversion occurred against viral antigens p24, p55 and gp68, while reactivity to p19 was variable between rabbits. Using the polymerase chain reaction (PCR) with HTLV\u2010I <jats:italic>gag<\/jats:italic> and <jats:italic>pol<\/jats:italic> primer pairs, HTLV\u2010I sequences were demonstrable in peripheral blood mononuclear cells and other tissues collected at 70 and 90 weeks after inoculation. DNA extracts from normal rabbit tissue remained negative under the same conditions. No qualitative or quantitative changes in leukocytes or erythrocytes were detected in the infected rabbits and no clinical signs could be directly attributed to infection with HTLV\u2010I.<\/jats:p>","DOI":"10.1002\/ijc.2910450123","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:00:18Z","timestamp":1171922418000},"page":"127-130","source":"Crossref","is-referenced-by-count":34,"title":["Persistent infection of rabbits with HTLV\u2010I: Patterns of anti\u2010viral antibody reactivity and detection of virus by gene amplification"],"prefix":"10.1002","volume":"45","author":[{"given":"Gary L.","family":"Cockerell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Lairmore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barun","family":"De","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel","family":"Rovnak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trudie M.","family":"Hartley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Miyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.6.1158"},{"key":"e_1_2_1_2_1","first-page":"86","article-title":"Experimental infection of rabbits with human T\u2010cell leukemia virus type I","volume":"76","author":"Akagi T.","year":"1985","journal-title":"Jpn. 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