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One hundred and two samples were chosen retrospectively and a further 70 consecutive patients were analysed prospectively in order to assess the prognostic significance of DNA ploidy and DNA index (D1). There were no statistically significant differences between retrospective and prospective groups in regard to age, sex, TNM stage, ploidy or D1. Sixty\u2010seven percent of patients were aneuploid (65% retrospective; 71% prospective). The proportion of aneuploid tumours was significantly higher among poorly differentiated tumours. Survival analysis using Cox multivariate regression modelling revealed that DNA aneuploidy and increasing D1 were significant independent prognostic factors for both relapse\u2010free and overall survival. The relapse and death rates among aneuploid subjects were approximately 3 times as high as those for diploid subjects. Patients with a D1 &gt;2.11 (hyper\u2010tetraploidy) experienced a 6.6\u2010fold higher death rate than diploid subjects. These results provide strong support for the incorporation of DNA ploidy profiles into the clinical management of patients with head and neck cancer.<\/jats:p>","DOI":"10.1002\/ijc.2910470107","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:08:06Z","timestamp":1171922886000},"page":"31-37","source":"Crossref","is-referenced-by-count":49,"title":["Prognostic importance of cellular dna content in head\u2010and\u2010neck squamous\u2010cell cancers. 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