{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:40:33Z","timestamp":1698126033859},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":5038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1992,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A spontaneously transformed rat\u2010liver epithelial\u2010cell line that could proliferate in unsupplemented, serum\u2010free medium and be passaged with trypsinization has been established. At the time of writing, the line has undergone more than 100 passages in serum\u2010free culture. This cell line produced an autocrine growth\u2010stimulatory factor (AGSF) and a paracrine growth\u2010stimulatory factor (PGSF). AGSF had a remarkable growth\u2010stimulatory effect on transformed rat\u2010liver epithelial\u2010cells, but had no such effect on non\u2010transformed rat\u2010liver epithelial\u2010cells that could not proliferate in serum\u2010free medium. AGSF did not show a growth\u2010inhibitory or stimulatory effect on normal rat kidney (NRK) cells in monolayer culture, and did not induce anchorage\u2010independent growth in soft\u2010agar culture even with epidermal growth factor (EGF) or transforming growth factor (TGF)\u2010p. AGSF was protease\u2010sensitive, but heat\u2010 and acid\u2010stable. The molecular weight was about 700 Dalton (Da) on size\u2010exclusion chromatography. PGSF showed a growth\u2010stimulatory effect on NRK cells and induced anchorage\u2010independent growth in soft\u2010agar culture without EGF or TGF\u2010\u03b2. On the other hand, PGSF slightly inhibited the growth of the spontaneously transformed rat\u2010liver epithelial cells. PGSF was heat\u2010, protease\u2010 and acid\u2010sensitive. The molecular weight was 30 kDa on size\u2010exclusion chromatography. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910520321","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T22:51:25Z","timestamp":1171925485000},"page":"455-460","source":"Crossref","is-referenced-by-count":2,"title":["Spontaneously transformed rat\u2010liver epithelial\u2010cell line producing autocrine and paracrine growth factors"],"prefix":"10.1002","volume":"52","author":[{"given":"Takeo","family":"Narita","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"2898","article-title":"Growth factor production by human colon carcinoma cell lines","volume":"49","author":"Anzano M. 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