{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T07:19:24Z","timestamp":1779261564085,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":4522,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The level of amplification (copy number\/cell) of HPV16 and HPV18 viral genomes and its correlation with the presence of E1\/E2 genes were analyzed in a sample of 42 HPV16\u2010and 21 HPV18\u2010positive cervical carcinomas of different clinical stages and histological types. The viral copy number\/cell was assessed by dot\u2010blot hybridization and the presence of E1\/E2 genes by PCR and Southern blot. The copy number\/cell was significantly lower in HPV18\u2010positive than in HPV16\u2010postive tumours (23 \u2213 8 and 457 \u2213 191 respectively). Nearly half of the HPV16s (43%) were distributed similarly to the HPV18s in the ranges of 50 or less copies, having its peak at the group of 1 to 10 copies, whereas the remaining HPV16s (57%) spread over the groups of 51 or more copies, with another peak at the group of 101 to 500. The E1\/E2 region was absent in all tumours positive for HPV18 and present in 64% of those positive for HPV16. The HPV16 tumours negative for E1 \/E2 had a much lower viral copy number (17 \u2213 12) than the positive ones (582 \u2213 212), thus resembling HPV18\u2010positive tumours. Viral copy number was negatively correlated with the clinical stage of the tumours and directly associated with the degree of histological differentiation. However, these correlations are primarily attributable to the presence or absence of an intact E1\/E2 region.<\/jats:p>","DOI":"10.1002\/ijc.2910560506","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:12:51Z","timestamp":1171930371000},"page":"640-645","source":"Crossref","is-referenced-by-count":59,"title":["Genome amplification of human papillomavirus types 16 and 18 in cervical carcinomas is related to the retention of E1\/E2 genes"],"prefix":"10.1002","volume":"56","author":[{"given":"Jaime","family":"Berumen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonora","family":"Casas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Segura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 L.","family":"Amezcua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Garcia\u2010Carranca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.5.2254-2260.1991"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90195-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90343-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1128\/jvi.65.2.606-612.1991","article-title":"Analysis of the physical state of different human papillomavirus DNAs in intraepithehal and invasive cervical neoplasm","volume":"65","author":"Cullen A. P.","year":"1991","journal-title":"J. Virol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.10.5949-5958.1992"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90068-Z"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90688-L"},{"key":"e_1_2_1_9_1","first-page":"275","article-title":"Human papillomaviruses and cervical cancer","volume":"5","author":"Gissmann L.","year":"1987","journal-title":"Cancer Cells"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90172-R"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(88)80070-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006250-199203000-00002"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90107-4"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)90353-9"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90829-Z"},{"key":"e_1_2_1_16_1","volume-title":"Molecular cloning. A laboratory manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910390611"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"3295","DOI":"10.1128\/jvi.61.10.3295-3298.1987","article-title":"Integration of human papillomavirus\u2010rype\u201016 DNA sequences: a possible early event in the progression of genital tumours","volume":"61","author":"Schneider\u2010Maunoury S.","year":"1987","journal-title":"J. Virol."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/314111a0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90214-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(92)90232-R"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07967.x"},{"key":"e_1_2_1_23_1","first-page":"781","article-title":"Human papillomavirus genotype as a prognostic indicator in carcinoma of the uterine cervix","volume":"74","author":"Walker J.","year":"1989","journal-title":"Obstet. Gynecol."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90539-9"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910560506","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910560506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T01:17:57Z","timestamp":1698110277000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910560506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,3]]}},"alternative-id":["10.1002\/ijc.2910560506"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910560506","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3]]}}}