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Amplification of the c\u2010<jats:italic>myc<\/jats:italic> gene was observed in 5 cell lines derived from well\u2010differentiated carcinomas and all of them were accompanied by co\u2010amplification of other examined oncogenes. The c\u2010<jats:italic>erb<\/jats:italic>B gene was amplified in 3 cell lines. Co\u2010amplification of <jats:italic>hst<\/jats:italic>\u20101 and <jats:italic>cyclin<\/jats:italic> DI, both of which are located in chromosome 11q13, was found in 9 cell lines. Without exception their amplification was simultaneous and the magnitudes were similar. Their amplification, but not their overexpression, was significantly correlated with poor prognosis in patients from whom the cell lines were established. While <jats:italic>hst<\/jats:italic>\u20101\u2010gene expression was not detected, at least 1 of the genes analyzed was overexpressed in 20 cell lines vs. its expression in normal esophageal mucosal tissues. However, gene amplification was not necessarily accompanied by overexpression of the corresponding genes. Expression of the <jats:italic>cyclin<\/jats:italic> DI gene, which has been assumed to be a target gene for 11q13 amplification, was not detected in one particular cell line with amplification of 11q13.<\/jats:p><jats:p>These results suggest that the amplification\/overexpression of more than I oncogene is involved in the carcinogenic process of esophageal carcinoma and that c\u2010<jats:italic>myc<\/jats:italic>\u2010gene amplification is associated with a well\u2010differentiated subtype. There remains a possibility that key oncogenes other than <jats:italic>cyclin<\/jats:italic> DI are involved in 11q13 amplification.<\/jats:p>","DOI":"10.1002\/ijc.2910580224","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:23:13Z","timestamp":1171930993000},"page":"291-297","source":"Crossref","is-referenced-by-count":56,"title":["Analysis of gene amplification and overexpression in human esophageal\u2010carcinoma cell lines"],"prefix":"10.1002","volume":"58","author":[{"given":"Yuji","family":"Kanda","sequence":"first","affiliation":[]},{"given":"Yasuyuki","family":"Nishiyama","sequence":"additional","affiliation":[]},{"given":"Yutaka","family":"Shimada","sequence":"additional","affiliation":[]},{"given":"Masayuki","family":"Imamura","sequence":"additional","affiliation":[]},{"given":"Hiroshi","family":"Nomura","sequence":"additional","affiliation":[]},{"given":"Hiroshi","family":"Hiai","sequence":"additional","affiliation":[]},{"given":"Manabu","family":"Fukumoto","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.6.1707"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/326833a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90136-E"},{"key":"e_1_2_1_6_1","first-page":"1693","article-title":"Association of Ki\u2010ras with amplified DNA sequences, detected in human ovarian carcinomas by a modified in\u2010gel renaturation assay","volume":"49","author":"Fukumoto M.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_1","first-page":"193","volume-title":"Molecular biology of cancer genes","author":"Fukumoto M.","year":"1990"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.6846"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01233951"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(92)90018-5"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711620403"},{"key":"e_1_2_1_12_1","first-page":"5119","article-title":"Amplification of epidermal\u2010growth\u2010factor\u2010receptor gene but no evidence of ras mutations in primary human esophageal cancers","volume":"48","author":"Hollstein M. 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