{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:23:11Z","timestamp":1759335791167},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4276,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1994,11]]},"abstract":"Abstract<\/jats:title>Mutations of the tumor\u2010suppressor gene TP53<\/jats:italic> and amplification of CCNDI<\/jats:italic> gene have been reported to occur frequently in head and neck squamous cell carcinomas (HNSQCC). In experimental systems, TP53<\/jats:italic> mutations have been shown to lead to genomic instability, including an increased propensity for gene amplification. We have examined I6 HNSQCC cell lines for the association between TP53<\/jats:italic> over\u2010expression\/mutation and CCNDI<\/jats:italic> amplification. p53<\/jats:italic> over\u2010expression was detected in 50% of the cell lines by immunohistochemistry using the monoclonal antibody (MAb) PAbI80l. TP53<\/jats:italic> mutations were also detected in 50% of the cell lines by analysis of single\u2010strand conformation polymorphism (SSCP) and DNA sequencing of exons 4 through 9. Six cell lines showed TP53<\/jats:italic> mutations and over\u2010expression of the protein, 2 cell lines showed TP53<\/jats:italic> mutations but no p53 expression, and 2 cell lines showed over\u2010expression of p53 protein but no TP53<\/jats:italic> gene mutations. CCNDI<\/jats:italic> amplification was found in 38% of the cell lines by Southern blot analysis. Only I cell line showed both TP53<\/jats:italic> mutation and CCNDI<\/jats:italic> amplification, whereas 7 of 8 cell lines with TP53<\/jats:italic> mutations had no CCNDI<\/jats:italic> amplification. pRb expression was detected by Western blot analysis, and the level of pRb did not correlate with either CCNDI<\/jats:italic> amplification or TP53<\/jats:italic> mutation. Our findings suggest that TP53<\/jats:italic> mutation and CCNDI<\/jats:italic> amplification are common genetic alterations in HNSQCC and that the occurrence of either genetic event may be sufficient to abrogate normal cell cycle control. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910590316","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:16:36Z","timestamp":1171930596000},"page":"383-387","source":"Crossref","is-referenced-by-count":27,"title":["TP53<\/i> gene mutations and CCND1<\/i> gene amplification in head and neck squamous cell carcinoma cell lines"],"prefix":"10.1002","volume":"59","author":[{"given":"Li","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J.","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. V. V. S.","family":"Murty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong\u2010Guo","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G.","family":"Sacks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Garin\u2010Chesa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stimson P.","family":"Schantz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S. K.","family":"Chaganti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.5.812"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09919.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90196-5"},{"key":"e_1_2_1_5_1","first-page":"1765","article-title":"Increased cyclin A and decreased cyclin D levels in adenovirus 5 E1A\u2010transformed rodent cell lines","volume":"8","author":"Buchou T.","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_1_6_1","first-page":"609","article-title":"Frameshift and nonsense p53 mutations in squamous cell carcinoma of head and neck: non\u2010reactivity with three anti\u2010p53 monoclonal antibodies","volume":"4","author":"Chen Y.\u2010T.","year":"1994","journal-title":"Int. J. 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