{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:50:28Z","timestamp":1757703028352},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":4213,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1995,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Trypsin inhibitors in serum\u2010free conditioned media (SFCM) of various human carcinoma cell lines were analyzed by reverse zymography. Most of the cells secreted high\u2010molecular\u2010weight trypsin inhibitors (HMTI) larger than 100 kDa. The cell lines of colorectal carcinoma origin had a tendency to secrete HMTI whose molecular weight was a little higher than that of the other cell lines. Analysis of SFCM of subclones with different histological differentiation and metastatic\/invasive potentials derived from a single pancreatic carcinoma cell line SUIT\u20102 showed that the HMTI activity in SFCM was correlated to the degree of histological differentiation <jats:italic>in vivo<\/jats:italic> and tended to be inversely correlated to their metastatic\/invasive capabilities. Immunoblotting analysis revealed that these HMTI were protease nexin\u2010ll\/amyloid \u03b2 protein precursors (PN\u2010II\/APP). Semi\u2010quantificative reverse\u2010transcriptase\/polymerase\u2010chain reaction study for PN\u2010II\/APP mRNAs suggested that the differences in PN\u2010II\/APP activities in SFCM between the subclones might be post\u2010transcriptional or post\u2010secretional events. In addition, SFCM of a highly metastatic subclone contained 43\u2010kDa protein which reacted to anti\u2010APP monoclonal antibody (MAb) suggesting that the subclone may have APP\u2010degrading activity. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910600118","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:42:20Z","timestamp":1171932140000},"page":"123-128","source":"Crossref","is-referenced-by-count":10,"title":["Reverse\u2010zymographic analysis of protease nexin\u2010II\/amyloid \u03b2 protein precursor of human carcinoma cell lines, with special reference to the grade of differentiation and metastatic phenotype"],"prefix":"10.1002","volume":"60","author":[{"given":"Hiroaki","family":"Kataoka","sequence":"first","affiliation":[]},{"given":"Kohji","family":"Seguchi","sequence":"additional","affiliation":[]},{"given":"Takeshi","family":"Iwamura","sequence":"additional","affiliation":[]},{"given":"Takuzou","family":"Moriyama","sequence":"additional","affiliation":[]},{"given":"Kazuki","family":"Nabeshima","sequence":"additional","affiliation":[]},{"given":"Masashi","family":"Koono","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"3239","article-title":"A rapid in vitro assay for quantitating the invasive potential of tumor cells","volume":"47","author":"Albini A.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(94)90173-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"4481","DOI":"10.1016\/S0021-9258(18)68951-3","article-title":"Alpha\u20101\u2010proteinase inhibitor is a neutrophil chemoattractant after proteolytic inactivation by macrophage elastase","volume":"263","author":"Banda M. 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