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We detected transcripts for 2 cytoplasmic (ABL and FES) and 6 receptor (ECK, ERB\u2010B2, FGF\u2010R4, IGFI\u2010R, KDR and TIE) kinases but not for receptors RET or TRK\u2010A. Genes for ECK, FGF\u2010R4 and TIE were expressed ectopically in melanomas (not in normal melanocytes). Similarly, ECK protein was detected by immunoblotting in metastatic melanomas but not in normal melanocytes. ECK mRNA levels tended to increase again during late melanoma progression. ECK and TIE mRNAs were also detected in highly metastatic variant cells but not in the corresponding poorly metastatic parental lines. Conversely, FES and KDR gene expression was lost in most advanced primary and metastatic melanomas. These findings suggest positive and negative roles for specific tyrosine kinases during progression. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ijc.2910600119","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T00:42:20Z","timestamp":1171932140000},"page":"129-136","source":"Crossref","is-referenced-by-count":70,"title":["Abnormal protein tyrosine kinase gene expression during melanoma progression and metastasis"],"prefix":"10.1002","volume":"60","author":[{"given":"David J.","family":"Easty","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meenhard","family":"Herlyn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy C.","family":"Bennett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3080-0_8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1991.tb00309.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/368558a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60383-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1991.375"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12371675"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3659911"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.6.2018"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1106"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-199210000-00002"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02623492"},{"key":"e_1_2_1_13_1","first-page":"2296","article-title":"In vitro properties of human melanoma cells metastatic in nude mice","volume":"50","author":"Herlyn D.","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_1_14_1","first-page":"461","article-title":"Biology of tumor progression in human melanocytes","volume":"56","author":"Herlyn M.","year":"1987","journal-title":"Lab. 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