{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T04:19:59Z","timestamp":1777781999714,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,3,20]],"date-time":"2009-03-20T00:00:00Z","timestamp":1237507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Development of cell\u2010patterning techniques is a major challenge for the construction of functional tissues and organs in tissue engineering. Recent progress in surface chemistry has enabled spatial control of cell adhesion onto cultural substrates by varying hydrophilicity, for example, by using poly (ethylene glycol) (PEG). In the present study, we developed a novel cell\u2010patterning procedure using PEG\u2010modified magnetite particles (PEG\u2010Mags) and magnetic force. Using an array\u2010patterned magnet, PEG\u2010Mags were magnetically patterned on the surface of a tissue culture dish. The resultant substrate surface consisted of two regions: the PEG\u2010Mag surface that acts as a cell\u2010resistant region and the native substrate surface that promotes cell adhesion. When human keratinocyte HaCaT cells were seeded onto the PEG\u2010Mag\u2010patterned surface, cells adhered only to the native substrate surface, resulting in cell\u2010patterning on the tissue culture dish. The patterned PEG\u2010Mags were then washed away to expose the native substrate surface, and thereafter, when mouse myoblast C2C12 cells were seeded to the dish, cells adhered to the exposed substrate surface, resulting in a patterned coculture of heterotypic cells. Moreover, it is worth noting that the magnetic force\u2010based cell\u2010patterning procedure is not limited by the property of cultural substrate surfaces, and that cell\u2010patterning of mouse fibroblast NIH3T3 cells on a monolayer of HaCaT cells was successfully achieved using PEG\u2010Mags and magnetic force. These results indicate that this procedure provides a novel concept for cell\u2010patterning and may be useful for tissue engineering and cell biology. \u00a9 2009 Wiley Periodicals, Inc. 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