{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:57:54Z","timestamp":1773302274760,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,11,29]],"date-time":"2011-11-29T00:00:00Z","timestamp":1322524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomedical Materials Res"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spinal cord injury (SCI) represents a major world health problem. Therefore it is urgent to develop novel strategies that can specifically target it. We have previously shown that the implantation of starch\u2010based scaffolds (SPCL) aimed for spine stabilization on SCI animals leads to motor skills improvements. Therefore, we hypothesize that the combination of these scaffolds with relevant cell populations for SCI repair will, most likely, lead to further improvements. Therefore, in this work, the ability of SPCL scaffolds to support the 3D culture of olfactory ensheathing cells (OECs) and Schwann cells (SCs) was studied and characterized. The results demonstrate for the first time that SPCL scaffolds were able to support the growth and migration of OECs and SCs. Moreover, the results indicate that two weeks of <jats:italic>in vitro<\/jats:italic> culture is the ideal time to reach a high number of transplantable cells. Future work will focus on the spine stabilization of SCI animals using SPCL scaffolds loaded with OECs or SCs for SCI regeneration. \u00a9 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.<\/jats:p>","DOI":"10.1002\/jbm.a.33289","type":"journal-article","created":{"date-parts":[[2011,11,30]],"date-time":"2011-11-30T10:27:16Z","timestamp":1322648836000},"page":"470-476","source":"Crossref","is-referenced-by-count":28,"title":["Interactions between Schwann and olfactory ensheathing cells with a starch\/polycaprolactone scaffold aimed at spinal cord injury repair"],"prefix":"10.1002","volume":"100A","author":[{"given":"Nuno A.","family":"Silva","sequence":"first","affiliation":[]},{"given":"Rui A.","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Ana O.","family":"Pires","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio J.","family":"Salgado","sequence":"additional","affiliation":[]},{"given":"Rui L.","family":"Reis","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2011,11,29]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"4","article-title":"Transfusions et greffes de moelle osseuse homologue chez des humaine irradies a haute dose accidentellement","author":"Math\u00e9 G","year":"1959","journal-title":"Nouvelle Rev Franc Hematol"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.sc.3101893"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awf014"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.b.10015"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3562-04.2005"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2007.0353"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.10.024"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2009.07.016"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-002-0607-y"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.11.002"},{"key":"e_1_2_5_12_2","first-page":"1488","article-title":"Influence of patients' age on functional recovery after transplantation of olfactory ensheathing cells into injured spinal cord injury","volume":"116","author":"Huang HY","year":"2003","journal-title":"Chin Med J"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/1545968305284675"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2004.06.027"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.3727\/000000004783983558"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2008.03.009"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0559"},{"key":"e_1_2_5_18_2","unstructured":"SilvaNA SousaRA OliveiraJT FragaJS FontesM CerqueiraR Leite\u2010AlmeidaH AlmeidaA SousaN ReisRL SalgadoAJ.Motor improvements after spine stabilization of spinal cord injured rats with a novel 3D natural based biodegradable scaffold. Proceedings of the XII Meeting of Portuguese Society for Neurosciences May 2011; Lisbon 206."},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(01)00044-4"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(87)90087-3"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.08.063"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2010.10.008"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2004.00262.x"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-24-10514.1998"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5334.2000"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awm155"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90134-N"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20166"},{"key":"e_1_2_5_29_2","doi-asserted-by":"crossref","first-page":"778","DOI":"10.2144\/04375ST03","article-title":"PicoGmeter, a custom\u2010made fluorometer for the quantification of dsDNA by PicoGreen (R) fluorescence","volume":"37","author":"Schofield GG","year":"2004","journal-title":"Biotechniques"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.3727\/000000002783985404"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-005-5134-3"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1152\/advan.00039.2002"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.04.061"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0470"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1367"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.182"},{"key":"e_1_2_5_37_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2007.0107"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X492616"},{"key":"e_1_2_5_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.11.033"},{"key":"e_1_2_5_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2009.01.039"},{"key":"e_1_2_5_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00346-X"},{"key":"e_1_2_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.07.014"},{"key":"e_1_2_5_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.07.045"}],"container-title":["Journal of Biomedical Materials Research Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbm.a.33289","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbm.a.33289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T21:14:14Z","timestamp":1697490854000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbm.a.33289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,29]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1002\/jbm.a.33289"],"URL":"https:\/\/doi.org\/10.1002\/jbm.a.33289","archive":["Portico"],"relation":{},"ISSN":["1549-3296","1552-4965"],"issn-type":[{"value":"1549-3296","type":"print"},{"value":"1552-4965","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,29]]}}}