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The presence of SV40 T\u2010ag, 53,000 (53K) cellular protein, and histocompatibility (H\u20102) antigens on the surface of SV40\u2010transformed cells was demonstrated by immunofluorescence. The use of lactoperoxidase\u2010catalyzed cell surface iodination and a differential immunoprecipitation technique established that large T\u2010ag is associated with the 53K host\u2010coded protein on the surface of the transformed cells. In contrast, no detergent\u2010stable complex between large T\u2010ag and H\u20102 antigens was detected. Both labeled T\u2010ag and 53K protein were coprecipitated from surface\u2010iodinated SV40\u2010transformed cells by monoclonal antibodies directed against either the viral or the cellular protein. Based on the unique antigenic sites recognized by the anti\u2010T monoclonal antibodies, it appears that both the carboxy and amino termini of the T\u2010ag polypeptide are exposed on the surface of SV40\u2010transformed mouse cells. The nature of the association between surface T\u2010ag and 53K protein, as well as that between the molecular complex and the plasma membrane, remains to be determined. The possible effect of the surface\u2010associated T\u2010ag\/53K complex on cellular proliferation is considered.<\/jats:p>","DOI":"10.1002\/jcb.240190204","type":"journal-article","created":{"date-parts":[[2004,12,31]],"date-time":"2004-12-31T08:28:56Z","timestamp":1104481736000},"page":"127-144","source":"Crossref","is-referenced-by-count":19,"title":["Detection of a complex of SV40 large tumor antigen and 53K cellular protein on the surface of SV40\u2010transformed mouse cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Myriam","family":"Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet 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