{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T05:14:25Z","timestamp":1719465265006},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":8084,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biological and biochemical properties of the transformation\u2010specific proteins of three avian oncornaviruses with different oncogenic potentials were compared, namely the <jats:italic>gag\u2010myc<\/jats:italic> protein of the avian myelocytomatosis virus MC29, the <jats:italic>gag\u2010erb A<\/jats:italic> protein of the avian erythroblastosis virus AEV, and the <jats:italic>gag\u2010fps<\/jats:italic> protein of Fujinami sarcoma virus FSV. These oncogenes were analyzed in transformed fibroblasts that expressed only the transforming proteins but showed no virus replication. Monoclonal antibodies against the viral structural protein p19, which is the N\u2010terminus of the proteins, were used for indirect immunofluorescence, for immunoprecipitation of the proteins from subcellular fractions, and for immunoaffinity column chromatography. With this last method a 3000\u2010fold purification of the proteins was obtained. By indirect immunofluorescence it was shown that the gag\u2010myc protein was located in the nucleus, and bound to DNA after purification. The <jats:italic>gag\u2010erb A<\/jats:italic> protein was not nuclear but probably located in the cytoplasm and did not bind to DNA after purification. Neither of the two proteins exhibited protein kinase activity. In contrast, the <jats:italic>gag\u2010fps<\/jats:italic> protein did not bind to DNA but showed protein kinase activity after purification. It was not located in the nucleus either.<\/jats:p>","DOI":"10.1002\/jcb.240200107","type":"journal-article","created":{"date-parts":[[2004,12,31]],"date-time":"2004-12-31T08:33:49Z","timestamp":1104482029000},"page":"63-69","source":"Crossref","is-referenced-by-count":8,"title":["Biochemical characterization of transformation\u2010specific proteins of acute avian leukemia and sarcoma viruses"],"prefix":"10.1002","volume":"20","author":[{"given":"K.","family":"Moelling","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. K.","family":"Owada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Greiser\u2010Wilke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Bunte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Donner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"217","volume":"91","author":"Hayward WS","year":"1981","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-52-1-1"},{"key":"e_1_2_1_4_2","first-page":"269","volume":"516","author":"Graf T","year":"1978","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_1_5_2","first-page":"3","volume-title":"Advances in Viral Research and Therapy","author":"Bister K","year":"1982"},{"key":"e_1_2_1_6_2","first-page":"214","volume-title":"Avian RNA Tumor Viruses","author":"Hayman MJ","year":"1978"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(79)90212-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90552-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1128\/jvi.36.2.617-621.1980","volume":"36","author":"Bister K","year":"1980","journal-title":"J Virol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1128\/jvi.39.1.325-329.1981","volume":"39","author":"Greiser\u2010Wilke I","year":"1981","journal-title":"J Virol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/296262a0"},{"key":"e_1_2_1_12_2","first-page":"25","volume":"91","author":"Erikson RL","year":"1981","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(77)90017-4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3514"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3484"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90361-1"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1128\/jvi.40.3.953-957.1981","volume":"40","author":"Coffin","year":"1981","journal-title":"J Virol"},{"key":"e_1_2_1_18_2","first-page":"5","volume":"53","author":"Tijan R","year":"1981","journal-title":"Curr Top Microbiol Immunol"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240200107","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240200107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T07:30:51Z","timestamp":1699774251000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240200107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,1]]}},"alternative-id":["10.1002\/jcb.240200107"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240200107","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,1]]}}}