{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:35:54Z","timestamp":1771461354637,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":7719,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1983,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rat PC12 pheochromocytoma and human A875 melanoma cells express nerve growth factor (NGF) receptors on their surfaces. Covalent crosslinking of bound <jats:sup>125<\/jats:sup>I\u2010NGF to PC12 or A875 intact cells or plasma membrane\u2010enriched fractions resulted in labelling of a peptide doublet at Mr = 110,000 and a single labelled peptide at Mr = 200,000 for each of the cell and membrane preparations. However, a difference between equilibrium binding properties of NGF\u2010receptor on PC12 and A875 cells was observed. PC12 cells exhibited biphasic binding properties with two apparent binding sites: K<jats:sub>D<\/jats:sub> = 5.2 nM sites and K<jats:sub>D<\/jats:sub> = 0.3 nM sites. The high\u2010affinity PC12 binding sites were trypsin resistant, and <jats:sup>125<\/jats:sup>I\u2010NGF dissociated slowly from them. A875 cells exhibited sites with homogeneous properties (K<jats:sub>D<\/jats:sub> = 1.0 nM), all binding sites were trypsin sensitive, and <jats:sup>125<\/jats:sup>I\u2010NGF dissociated rapidly in the presence of unlabelled NGF. Membrane\u2010enriched fractions from either cell type contained binding sites with a uniform low affinity (K<jats:sub>D<\/jats:sub> = 3 nM) that were trypsin sensitive, and <jats:sup>125<\/jats:sup>I\u2010NGF rapidly dissociated from them. Sixty to 80 percent of binding sites in membranes could be converted to the high\u2010affinity, trypsin\u2010resistant state by addition of wheat germ agglutinin (WGA). The loss of high\u2010affinity, trypsin\u2010resistant sites from PC12 cells during preparation of plasma membrane fractions does not appear to be the result of selective isolation of low\u2010affinity sites or proteolytic degradation since there is a loss of <jats:sup>125<\/jats:sup>I\u2010NGF binding immediately after cell lysis which is not blocked by protease inhibitors. Also, high\u2010affinity, trypsin\u2010resistant binding sites are not found associated with other cell fractions. The differences between receptor properties on PC12 cells and on A875 cells apparently are the result of differences in the respective intracellular environments. Thus, significant structural homology exists between receptors on A875 and PC12 cells. Cell components other than the binding unit of the NGF receptor may be responsible for the different properties of receptor.<\/jats:p>","DOI":"10.1002\/jcb.240220404","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T07:23:29Z","timestamp":1104391409000},"page":"219-233","source":"Crossref","is-referenced-by-count":38,"title":["A comparison of binding properties and structure of NGF receptor on PC12 pheochromocytoma and A875 melanoma cells"],"prefix":"10.1002","volume":"22","author":[{"given":"Stephen E.","family":"Buxser","sequence":"first","affiliation":[]},{"given":"Leslie","family":"Watson","sequence":"additional","affiliation":[]},{"given":"Gary L.","family":"Johnson","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.03.030180.002033"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50507-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.7.3242"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(79)90445-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.2.565"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.8.4751"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90112-4"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.94.3.710"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1269"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"9338","DOI":"10.1016\/S0021-9258(19)70567-5","volume":"255","author":"Olender EJ","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.1016\/S0021-9258(18)32727-3","volume":"258","author":"Buxser SE","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00670a019"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1680187"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.2.875"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"3370","DOI":"10.1016\/S0021-9258(18)32870-9","volume":"258","author":"Puma P","journal-title":"J Biol Chem"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240220404","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240220404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T10:47:48Z","timestamp":1697712468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240220404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,1]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1983,1]]}},"alternative-id":["10.1002\/jcb.240220404"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240220404","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,1]]}}}