{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:39:32Z","timestamp":1770475172703,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":5496,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1989,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The c\u2010erbB\u20102 proto\u2010oncogene is amplified in a high percentage of primary human breast tumors, suggesting that the overexpression of this gene may be involved in the development of human breast cancer. We have investigated five human breast tumor cell lines and have detected amplified c\u2010erbB\u20102 gene copies in two of them. This amplification leads to overexpression of the c\u2010erbB\u20102 protein. In addition, two other cell lines have elevated protein levels without gene amplification, suggesting that other mechanisms can lead to overexpression of the c\u2010erbB\u20102 protein. These results are similar to those that we obtained during a study of primary breast tumors (Berger et al.: Cancer Res 48:1238\u20131243, 1988). These breast tumor cell lines should be useful for an analysis of c\u2010erbB\u20102 expression and of the mechanisms that in some cases lead to overexpression.<\/jats:p>","DOI":"10.1002\/jcb.240390208","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T15:23:15Z","timestamp":1104420195000},"page":"167-173","source":"Crossref","is-referenced-by-count":79,"title":["Overexpression of the c\u2010erbB\u20102 protein in human breast tumor cell lines"],"prefix":"10.1002","volume":"39","author":[{"given":"Nancy E.","family":"Hynes","sequence":"first","affiliation":[]},{"given":"Heinz A.","family":"Gerber","sequence":"additional","affiliation":[]},{"given":"Suzanne","family":"Saurer","sequence":"additional","affiliation":[]},{"given":"Bernd","family":"Groner","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3541204"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60201-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6719137"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6497"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2992089"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2999974"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/312513a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2885917"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.20.7159"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.5.2019"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92736-X"},{"key":"e_1_2_1_14_2","first-page":"1238","volume":"48","author":"Berger MS","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb04797.x"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00709a025"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/319230a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02794.x"},{"key":"e_1_2_1_19_2","first-page":"259","volume":"2","author":"Redmond SMS","year":"1988","journal-title":"Oncogene"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910400221"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/29.4.6166661"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/307521a0"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.13.4374"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.7.2597"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.001205"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80050-7"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240390208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240390208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T12:35:45Z","timestamp":1697978145000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240390208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,2]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,2]]}},"alternative-id":["10.1002\/jcb.240390208"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240390208","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,2]]}}}