{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T02:11:53Z","timestamp":1775355113203,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":4981,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The uncoupling protein (UCP) gene encodes a unique mammalian mitochondrial proton carrier that induces heat production in brown adipocytes. Human UCP gene was isolated and its organization analyzed. A comparison was made with rat UCP gene. Human UCP gene spans 13 Kb and contains a transcribed region that covers 9 Kb of the human genome. All of the exons were also sequenced except the extreme end of the 3\u2032 untranslated region. Two Kb DNA upstream the TATA box were also sequenced. This region contains several fragments that are highly homologous to the gene of rat UCP. Neither CCAAT sequence nor Sp I binding motif were detected. Human UCP gene is split into six exons. The complete amino acid sequence of the protein was determined. Human UCP has 305 amino acids and a molecular weight of 32,786. It has no N\u2010terminal targeting sequence. It is 79% homologous to rat UCP both at nucleotidic and amino acid levels. The primary structure of UCP is significantly homologous to the primary structure of the human T<jats:sub>1<\/jats:sub> ADP\/ATP carrier, particularly in the C\u2010terminal extremity, which is supposed to contain a nucleotide\u2010 binding site in both proteins. Human UCP gene is single type, as it is in rodents. Two genomic fragments were used to detect a 1.9 Kb mRNA in human perirenal brown adipose tissue. Using in situ hybridization, UCP gene was assigned in humans to chromosome 4 in q31. Interestingly, the Tl gene encoding the heart\u2010skeletal muscle ADP\/ATP carrier has recently been shown to be on the same chromosome (Li et al. Biol Chem 264:13998, 1989).<\/jats:p>","DOI":"10.1002\/jcb.240430306","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T20:30:45Z","timestamp":1117312245000},"page":"255-264","source":"Crossref","is-referenced-by-count":103,"title":["Human uncoupling protein gene: Structure, comparison with rat gene, and assignment to the long arm of chromosome 4"],"prefix":"10.1002","volume":"43","author":[{"given":"Anne\u2010Marie","family":"Cassard","sequence":"first","affiliation":[]},{"given":"Fr\u00e9d\u00e9ric","family":"Bouillaud","sequence":"additional","affiliation":[]},{"given":"Marie\u2010Genevi\u00e8ve","family":"Mattei","sequence":"additional","affiliation":[]},{"given":"Eliane","family":"Hentz","sequence":"additional","affiliation":[]},{"given":"Serge","family":"Raimbault","sequence":"additional","affiliation":[]},{"given":"Muriel","family":"Thomas","sequence":"additional","affiliation":[]},{"given":"Daniel","family":"Ricquier","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1984.64.1.1"},{"key":"e_1_2_1_3_2","first-page":"110","volume":"20","author":"Cannon B","year":"1985","journal-title":"Essays Biochem"},{"key":"e_1_2_1_4_2","first-page":"86","volume-title":"Brown Adipose Tissue","author":"Ricquier D","year":"1986"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.2.445"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.10.4025"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)36228-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03941.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0021-9258(17)35962-8","volume":"261","author":"Bouillaud F","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02377.x"},{"key":"e_1_2_1_11_2","unstructured":"RicquierD BouillaudF CasteillaL CassardAM RaimbaultS KlausS ChampignyO HentzE: In Bray G Ricquier D Spiegelman B (eds): \u201cObesity: Towards a Molecular Approach.\u201d UCLA Symposia Proceedings 1989 in press."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37751-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80318-8"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/cs0750021"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293038"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61355-9"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7580"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.2.377"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"13998","DOI":"10.1016\/S0021-9258(19)85001-9","volume":"264","author":"Kang L","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-74539-3_20"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.5.2131"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00414a029"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00428a069"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(89)90477-4"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2570665"},{"key":"e_1_2_1_27_2","first-page":"12","volume":"78","year":"1987","journal-title":"Mouse News Letter: Oxford University Press"},{"key":"e_1_2_1_28_2","first-page":"132","volume":"46","author":"Gusella JF","year":"1987","journal-title":"Human Gene Mapping 9. 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