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Although the PKC\u2010\u03be cDNA encoded for 592 amino acids, a 76 kD protein was also generated by in vitro transcription\/translation. Extracts of cells expressing PKC\u2010\u03b4 were able to bind phorbol ester to levels comparable to extracts of cells expressing PKC\u2010\u03b1. No phorbol ester binding was, however, detected in insect cell extracts expressing PKC\u2010\u03be. However, similar levels of protein kinase activity were detected in lysates of cells expressing PKC\u2010\u03b4 or PKC\u2010\u03be when protamine sulfate was used as exogenous substrate. Compared to protamine sulfate, both, myelin basic protein (MBP) or histone, were poor substrates for PKC\u2010\u03b4 and PKC\u2010\u03be. In contrast to PKC\u2010\u03be, the PKC\u2010\u03b4 enzyme activity phosphorylated MBP or histone in a phosphatidylserine\u2010(PS)\/diacylglycerol(DG)\u2010dependent manner, albeit not to the same extent as PKC\u2010\u03b1. Lack of stimulation of the enzyme activity of PKC\u2010\u03be by PS\/DG, was confirmed by endogenous phosphorylation of insect cell proteins by PKC\u2010\u03be, whereas several insect cell proteins were phosphorylated by PKC\u2010\u03b4 in a PS\/DG\u2010dependent manner, including a protein of 78 kD.<\/jats:p><jats:p>Our data demonstrate that the 76 kD PKC\u2010\u03be, in contrast to PKC\u2010\u03b4, is unable to bind phorbol esters and displays a protein kinase activity that is independent of PS of PS\/DG. In addition, staurosporine was about 2\u20134 order of magnitudes less effective in inhibiting the protein kinase activities of PKC\u2010\u03b4 and PKC\u2010\u03b4 when compared to PKC\u2010\u03be.<\/jats:p>","DOI":"10.1002\/jcb.240490306","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T20:58:40Z","timestamp":1117313920000},"page":"239-250","source":"Crossref","is-referenced-by-count":54,"title":["Expression and partial characterization of rat protein kinase C\u2010\u03b4 and protein kinase C\u2010\u03be in insect cells using recombinant baculovirus"],"prefix":"10.1002","volume":"49","author":[{"given":"E.","family":"McGlynn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Liebetanz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Reutener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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