{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T10:36:41Z","timestamp":1779100601958,"version":"3.51.4"},"reference-count":63,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3277,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Steroid receptors have been reported to bind to the nuclear matrix. The nuclear matrix is operationally defined as the residual nuclear structure that remains after extraction of most of the chromatin and all soluble and loosely bound componnets. To obtain insight in the molecular mechanism of the interaction of steroid receptors with the nuclear matrix, we studied the binding of several deletion mutants of the human androgen receptor (hAR) and the human glucocorticoid receptor (hGR) to the nuclear matrix. Receptor binding was tested for two different nuclear matrix preparations: complete matrices, in which most matrix proteins are retained during the isolation procedure, and depleted matrices, which consist of only a subset of these proteins. The results show that the C\u2010terminal domain of the hAR binds tightly to both depleted and complete matrices. In addition, at least one other domain of the hAR binds to complete matrices but not to depleted matrices. In contrast to the hAR, the hGR binds only to complete matrices. For this interaction both the DNA\u2010binding domain and the C\u2010terminal domain of the hGR are required, whereas the N\u2010terminal domain is not. We conclude that specific protein domains of the hAR and the hGR are involved in binding to the nuclear matrix. In addition, our results indicate that the hAR and the hGR are attached to the nuclear matrix through different molecular interactions.<\/jats:p>","DOI":"10.1002\/jcb.240570312","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T22:07:11Z","timestamp":1117318031000},"page":"465-478","source":"Crossref","is-referenced-by-count":83,"title":["Domains of the human androgen receptor and glucocorticoid receptor involved in binding to the nuclear matrix"],"prefix":"10.1002","volume":"57","author":[{"given":"Bas","family":"van Steensel","sequence":"first","affiliation":[]},{"given":"Guido","family":"Jenster","sequence":"additional","affiliation":[]},{"given":"Klaus","family":"Damm","sequence":"additional","affiliation":[]},{"given":"Albert O.","family":"Brinkmann","sequence":"additional","affiliation":[]},{"suffix":"Dr.","given":"Roel","family":"van Driel","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.24.11660"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-120-5-1851"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-113-1-430"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1515\/9783110850260-004","volume-title":"Recent Advances in Steroid Hormone Action","author":"Barrack ER","year":"1987"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1016\/S0021-9258(20)79697-3","article-title":"The specific binding of estrogens and androgens to the nuclear matrix of sex hormone responsive tissues","volume":"255","author":"Barrack ER","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(89)90011-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240470204"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(74)90355-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3162"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.130.6.1597154"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(92)90352-9"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00263a018"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00310a015"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/jcs.90.1.1","article-title":"The nucleoskeleton: Artefact, passive framework or active site?","volume":"90","author":"Cook PR","year":"1988","journal-title":"J Cell Sci"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/39.8.1856453"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0309-1651(90)90014-P"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90184-W"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90030-3"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3283939"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(92)90258-K"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-9-1336"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-11-3-399"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240470402"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(88)90108-4"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950140905"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.3.569"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90145-6"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90753-7"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90256-4"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03719.x"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/292552a0"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05747.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-5-10-1396"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2930761"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(84)90208-8"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90088-4"},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"11962","DOI":"10.1016\/S0021-9258(18)67187-X","article-title":"Binding of the glucocorticoid receptor to the rat liver nuclear matrix","volume":"261","author":"Kaufmann SH","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91126-5"},{"key":"e_1_2_1_40_1","first-page":"545","article-title":"Interaction of myb proteins with nuclear matrix in vitro","volume":"2","author":"Klempnauer K\u2010H","year":"1988","journal-title":"Oncogene"},{"key":"e_1_2_1_41_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1042\/bj2910095","article-title":"Localization and hormonal stimulation of phosphorylation sites in the LNCaP\u2010cell androgen receptor","volume":"291","author":"Kuiper GGJM","year":"1993","journal-title":"Biochem J"},{"key":"e_1_2_1_42_1","doi-asserted-by":"crossref","first-page":"2844","DOI":"10.1016\/S0021-9258(17)35864-7","article-title":"Association of the 3,5,3\u2032\u2010triiodo\u2010L\u2010thyronine nuclear receptor with the nuclear matrix of cultured growth hormone\u2010producing rat pituitary tumor cells (GC cells)","volume":"261","author":"Kumara\u2010Siri MH","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90332-1"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.3.1087"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-4-2190"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90208-3"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90135-L"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.6.1609"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8382376"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(85)90451-3"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.7.4.597"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05404.x"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00103a019"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240470403"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.101.4.773"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(92)90174-7"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_58_1","first-page":"459","article-title":"Steroid hormone receptors\u2014interaction with deoxyribonucleic acid and transcription factors","volume":"14","author":"Truss M","year":"1993","journal-title":"Endocr Rev"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240470405"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80873-2"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00084a003"},{"key":"e_1_2_1_62_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1242\/jcs.90.1.11","article-title":"The nuclear matrix: Structure and composition","volume":"90","author":"Verheijen R","year":"1988","journal-title":"J Cell Sci"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.2.283"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(91)90178-J"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.240570312","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.240570312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T08:17:09Z","timestamp":1698308229000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.240570312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1002\/jcb.240570312"],"URL":"https:\/\/doi.org\/10.1002\/jcb.240570312","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]}}}