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Cell components were segregated in centrifuge fractions by a mild procedure (sucrose medium), which preserved mitochondria and vesicular inclusions, and by a drastic procedure, designed to preserve the striated borders selectively. The contents of fractions were characterized by phase contrast and electron microscopy and by the assay of alkaline phosphatase (E.C.3.1.3.1), cytochome oxidase (E.C.1.9.3.1), invertase (E.C.3.2.1.26) and Mg\u2010ATPase (E.C.3.6.1.4).<\/jats:p><jats:p>Na\u2010K ATPase was found to be most concentrated in fractions containing mitochondria on one hand, and striated borders on the other. Its distribution differed from the distributions of the other four enzymes. The physiological implications of finding the \u201csodium pump\u201d enzyme in the membrane at the apical pole of the epithelial cell were discussed.<\/jats:p>","DOI":"10.1002\/jcp.1030650309","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T17:03:51Z","timestamp":1109351031000},"page":"361-372","source":"Crossref","is-referenced-by-count":28,"title":["The sodium and potassium activated ATPase of intestinal epithelium. I. Location of enzymatic activity in the cell"],"prefix":"10.1002","volume":"65","author":[{"given":"G. G.","family":"Berg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Chapman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.17.1.1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(62)90282-5"},{"key":"e_1_2_1_4_1","unstructured":"Berg G. G. andJ.Szekerczes1965The sodium and potassium activated ATPase II. Comparative study of intestinal epithelium and red cells. 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