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Exchange diffusion was not observed, Phytohemagglutinin (PHA) significantly stimulated calcium uptake within minutes after treatment. The increased uptake was attributed to a decreased K<jats:sub>m<\/jats:sub> for the proposed membrane carrier rather than to an increased V<jats:sub>max<\/jats:sub>. Also PHA did not stimulate a normally unused exchange diffusion process, nor did it affect calcium efflux. Uptake by both unstimulated and PHA\u2010treated lymphocytes was not influenced by magnesium or by cycloheximide or actinomycin D.<\/jats:p>","DOI":"10.1002\/jcp.1040820103","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T18:11:14Z","timestamp":1109441474000},"page":"9-19","source":"Crossref","is-referenced-by-count":75,"title":["Characteristics of calcium accumulation by lymphocytes and alterations in the process induced by phytohemagglutinin"],"prefix":"10.1002","volume":"82","author":[{"given":"Richard B.","family":"Whitney","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Sutherland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"509","article-title":"The early uptake of radioactive calcium by human lymphocytes treated with phytohemagglutinin","volume":"21","author":"Allwood G.","year":"1971","journal-title":"Immunology"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.2.271"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.36.3.567"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.53.1.43"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.53.1.57"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/226945a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/214667a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.57.2.202"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1016\/S0021-9258(19)73792-2","article-title":"Amino acid transport in lymph node cells","volume":"237","author":"Helmreich W.","year":"1962","journal-title":"J. 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