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Serum was fractioned into a platelet\u2010derived growth factor (PDGF), which induces cells to become competent to synthesize DNA, and plasma which allows competent cells to traverse G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub> and enter the S phase. Nutrients are not required for the cellular response to PDGF; however amino acids are required for plasma to promote the entry of PDGF\u2010treated, competent cells into S phase. The nutrient independent, PDGF\u2010modulated, growth regulatory event (competence) is located 12 hours prior to the G<jats:sub>1<\/jats:sub>\/S phase boundary in quiescent, density\u2010arrested Balb\/c\u20103T3 cells. The nutrient dependent, plasma\u2010modulated event is located six hours prior to the G<jats:sub>1<\/jats:sub>\/S phase boundary and corresponds in time to a plasma dependent growth arrest point. Moreover, plasma controls the concentration of amino acids required for DNA synthesis. Infection of density\u2010arrested Balb\/c\u20103T3 cells with SV40 overrides both the nutrient independent and the nutrient dependent growth regulatory events.<\/jats:p>","DOI":"10.1002\/jcp.1040990314","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:18:13Z","timestamp":1109434693000},"page":"395-405","source":"Crossref","is-referenced-by-count":87,"title":["Control of the Balb\/c\u20103T3 cell cycle by nutrients and serum factors: Analysis using platelet\u2010derived growth factor and platelet\u2010poor plasma"],"prefix":"10.1002","volume":"99","author":[{"given":"C. D.","family":"Stiles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. R.","family":"Isberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Pledger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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