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The synthesis of MT can also be induced by either heavy metals or glucocorticoid hormones in HeLa cells cultured in serum\u2010free medium. Induction of MT by zinc is subject to \u201cdesensitization.\u201d In contrast, dexamethasone (dex) induction results in a continued elevation in the rate of MT synthesis. The stability of MT is dependent on the availability of metal; consequently, MT induced by dex is degraded much more rapidly (half\u2010life of 11 to 12 hours) than MT induced by elevated zinc levels (half\u2010life of 36 to 38 hours). Removal of either inducer results in biphasic degradation curves, as apothionein and zinc come into balance. In contrast, deinduction kinetics for MT synthesis following removal of the two inducers (zinc and dex) are the same, with a half\u2010life of two and one\u2010half hours. Inhibition of RNA synthesis blocks deinduction after removal of inducer. Induction of MT occurs in a wide variety of species, from blue\u2010green algae to man. This system should provide an excellent model for the comparative biochemistry of regulation of gene expression.<\/jats:p>","DOI":"10.1002\/jcp.1041060108","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T16:09:53Z","timestamp":1109434193000},"page":"63-74","source":"Crossref","is-referenced-by-count":44,"title":["Regulation of metallothionein synthesis in HeLa cells by heavy metals and glucocorticoids"],"prefix":"10.1002","volume":"106","author":[{"given":"Michael","family":"Karin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily P.","family":"Slater","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey R.","family":"Herschman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1750841"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0010982"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1490733"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00699a013"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.6.2669"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1093\/jn\/107.6.965","article-title":"Studies on the synthesis and metabolism of zinc\u2010thionein in rats","volume":"107","author":"Cherin M. 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