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In serum\u2010containing medium the maximal level of cell\u2010bound EGF was maintained for at least 8 h without appreciable degradation in contrast to serum\u2010free conditions. These phenomena were correlated with a difference in the intracellular site to which the receptor\u2010bound EGF was delivered as studied using Percoll density gradients. In serum\u2010containing medium the majority of cell\u2010bound EGF was initially taken up into a Golgi\u2010like vesicle of density 1.046, corresponding to the marker galactosyl transferase, and then delivered to a population of vesicles with similar density as lysosomes (\u03f1 =1.068\u20131.110). A portion of the EGF became degraded and was released from the cell into the medium while the remainder stayed with the cells, intact, for a long period of time. In serum\u2010free medium, EGF became associated with a heterogeneous population of vesicles with a mean density of 1.050 which do not correspond to any of the marker enzymes for subcellular organelles for which we have tested (Golgi, endoplasmic reticulum, plasma membrane, lysosomes). It is then transferred to lysosome\u2010like vesicles (\u03f1 = 1.068\u20131.110). We therefore propose that EGF is processed through two separate endocytotic routes which are regulated by the cell depending upon its physiological state.<\/jats:p>","DOI":"10.1002\/jcp.1041120304","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T15:27:22Z","timestamp":1109431642000},"page":"327-338","source":"Crossref","is-referenced-by-count":39,"title":["Dual pathways for epidermal growth factor processing after receptor\u2010mediated endocytosis"],"prefix":"10.1002","volume":"112","author":[{"given":"W. 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