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MDCK cells were found to transport neutral amino acids by systems similar to the A, ASC, L, and N systems which have been characterized using other cell lines. Experimental conditions were developed for MDCK cells which allowed independent measurement of A, ASC, and L transport activities. The activity of the L system was measured as Na<jats:sup>+<\/jats:sup>\u2010independent leucine or methionine uptake at pH 7.4. The activity of the A system was measured as Na<jats:sup>+<\/jats:sup>\u2010dependent \u03b1(methylamino)isobutyric acid (mAIB) uptake at pH 7.4, the activity of the ASC system was measured as Na<jats:sup>+<\/jats:sup>\u2010dependent alanine uptake in the presence of 0.1 mM mAIB at pH 6.0, and the activity of system N was observed by measuring Na<jats:sup>+<\/jats:sup>\u2010dependent glutamine uptake at pH 7.4 in the presence of high concentrations of A and ASC system substrates. The L transport system responded minimally to changes in growth state, but Na<jats:sup>+<\/jats:sup>\u2010dependent amino add transport responded to regulation by growth factors, cell density, and transformation. The activities of the A and ASC systems both decreased at high cell density, but these activities responded dissimilarly under other conditions. The activity of the A system was stimulated by insulin, was inhibited by PGE<jats:sub>1<\/jats:sub>, and was elevated 3\u20137 fold in the transformed cell line, MDCK\u2010T<jats:sub>1<\/jats:sub>. The activity of the ASC system was slightly stimulated by insulin and by PGE<jats:sub>1<\/jats:sub>, but was unchanged after chemical transformation. Changes in cellular growth were monitored and were found to correlate best with the activity of the A system. These results suggested that MDCK cell growth may be more closely related to the activity of the A than of the ASC system.<\/jats:p>","DOI":"10.1002\/jcp.1041130209","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:54:29Z","timestamp":1109429669000},"page":"240-246","source":"Crossref","is-referenced-by-count":57,"title":["Growth regulation and amino acid transport in epithelial cells: Influence of culture conditions and transformation on A, ASC, and l transport activities"],"prefix":"10.1002","volume":"113","author":[{"given":"Paula","family":"Boerner","sequence":"first","affiliation":[]},{"suffix":"Jr.","given":"Milton H.","family":"Saier","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"10259","DOI":"10.1016\/S0021-9258(19)68613-8","article-title":"The A, ASC, and L systems for the transport of amino acids in Chinese hamster ovary cells (CHO\u2010K1)","volume":"256","author":"Bass R.","year":"1981","journal-title":"J. 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