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Previous suggestions that the phosphorylation of a 44,000 dalton membrane protein is involved in this permeabilization process have been pursued. Fractionation of cells that had been incubated with [\u03b3\u2010<jats:sup>32<\/jats:sup>P] ATP revealed that the labeled 44K phosphoprotein was found in both the membrane and mitochondrial fractions. Incubation of fractions isolated from unlabeled cells with [\u03b3\u2010<jats:sup>32<\/jats:sup>P] ATP resulted in substantial formation of <jats:sup>32<\/jats:sup>P\u201044K in the mitochondrial fraction and less incorporation in the membrane fraction. The 44,000 dalton protein was identified as the \u03b1\u2010subunit of mitochondrial pyruvate dehydrogenase by partial proteolytic mapping and immunological cross\u2010reactivity with antibodies prepared against bovine pyruvate dehydrogenase. The phosphorylation of this protein in whole cells by externally added ATP is suppressed by inclusion in the incubation medium of carboxyatractyloside (CAT) and EDTA. These substances have no effect on ATP\u2010dependent permeabilization, indicating that the phosphorylation of pyruvate dehydrogenase is not involved in this process.<\/jats:p>","DOI":"10.1002\/jcp.1041180204","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T14:51:49Z","timestamp":1109429509000},"page":"124-132","source":"Crossref","is-referenced-by-count":19,"title":["On the role of protein phosphorylation in the ATP\u2010dependent permeabilization of transformed cells"],"prefix":"10.1002","volume":"118","author":[{"given":"Gary A.","family":"Weisman","sequence":"first","affiliation":[]},{"given":"Stanley D.","family":"Dunn","sequence":"additional","affiliation":[]},{"given":"Barun K.","family":"De","sequence":"additional","affiliation":[]},{"given":"Takayuki","family":"Kitagawa","sequence":"additional","affiliation":[]},{"given":"Ilan","family":"Friedberg","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00648a026"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(66)81051-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870550"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"6854","DOI":"10.1016\/S0021-9258(19)42137-6","article-title":"Cyclic adenosine monophosphate\u2010dependent phosphorylation of specific fat cell membrane proteins by an endogenous membrane\u2010bound protein kinase: Possible involvement in the regulation of insulin\u2010stimulated glucose transport","volume":"249","author":"Chang K. 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