{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T14:30:22Z","timestamp":1698071422414},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":7219,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1985,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has previously been shown that fibroblastic cells transformed by SV40 exhibit a reduced requirement for PDGF for growth. In addition, NIH\/3T3 cells lose both their chemotactic response to PDGF and specific cell surface binding of PDGF after transformation with SV40. We have now examined whether the SV40 transformed NH\/3T3 cells are producing a factor which acts similarly to PDGF. Our studies indicate that NIH\/3T3 cells transformed with SV 40 produce a factor which shares many biological properties with PDGF. We were unable to detect this activity in conditioned media from non\u2010transformed NIH\/3T3 cells. The SV40\/NIH\/3T3 derived factor appears to possess both chemotactic and mitogenic activity for connective tissue cells but not endothelial or epithelial cells. Furthermore, in preliminary studies, this activity competes with <jats:sup>125<\/jats:sup>I\u2010PDGF for binding to smooth muscle cells. The biochemical properties of the SV40\/NIH\/3T3 derived factor are different from those of PDGF. The SV40 activity appears to reside in a heat labile acidic protein (pI &lt; 7.0) of MW &lt; 30,000 whereas PDGF is a heat stable basic protein (pI9.8) of 30,000 MW. Production of this factor may play a role in the decreased serum requirement for cell replication exhibited by SV40\u2010transformed NIH\/3T3 cells by supplying the cells with their own PDGF\u2010like growth factor.<\/jats:p>","DOI":"10.1002\/jcp.1041230203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T13:51:09Z","timestamp":1109425869000},"page":"161-166","source":"Crossref","is-referenced-by-count":14,"title":["Identification of a PDGF\u2010like mitoattractant produced by NIH\/3T3 cells after transformation with SV40"],"prefix":"10.1002","volume":"123","author":[{"given":"Ilan","family":"Bleiberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita K.","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgeann","family":"Smale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Grotendorst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.7.2635"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.4.1809"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.20.6264"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/309804a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2396"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1981.53"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.4001"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6310754"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(81)90314-1"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6304883"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/227802a0"},{"key":"e_1_2_1_13_1","first-page":"83","article-title":"Evidence that a human osteosarcoma cell line which secretes a mitogen similar to platelet\u2010derived growth factor requires growth factors present in platelet\u2010poor plasma","volume":"43","author":"Graves D. 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