{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:04:20Z","timestamp":1774620260489,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6458,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1987,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Survival after H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> exposure or heat shock of asynchronous Chinese hamster ovary cells (HA\u20101) was assayed following pretreatment with mildly toxic doses of either H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> or hyperthermia. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> cytotoxicity at 37\u00b0C, expressed as a function of mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> was found to be dependent on cell density at the time of treatment. The density dependence reflected the ability of cells to reduce the effectiveness of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> as a cytotoxic agent. When the survival data were plotted as a function of \u03bcmoles H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/cell at the beginning of the treatment, survival was independent of cell density. Cells pretreated with 0.1 mM (3\u20135 \u03bcmoles\/cell \u00d7 10<jats:sup>\u22127<\/jats:sup>) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> for 1 hr at 37\u00b0C (30\u201350% survival) became resistant to a subsequent H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> treatment 16\u201336 hr after pretreatment [dose modifying factor (DMF) at 1% isosurvival = 4\u20136]. Their resistance to 43\u00b0C heating, however, was only slightly increased over controls 16\u201336 hr following pretreatment (DMF at 1% isosurvival = 1.2). During this same interval, the synthesis of protein migrating in the 70 kD region of a one\u2010dimensional SDS\u2010polyacrylamide gel was enhanced twofold in the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010pretreated cells. When the cells were heated for 15 min at 45\u00b0C (40\u201360% survival), the survivors became extremely resistant to 43\u00b0C heating and somewhat resistant to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> (DMF at 1% isosurvival = 2). The heat\u2010induced resistance to heat developed much more rapidly (reached a maximum between 6 and 13 hr) following pretreatment than the heat\u2010induced rsistance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> (16\u201336 hr). The enhanced synthesis of 70 kD protein after heat shock was greater in magnitude and occurred more rapidly following preheating than following H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> pretreatment. The cells that became resistant to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> by either pretreatment (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> or heat shock) also increased their ability to reduce the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> cytotoxicity from the treatment medium beyond that of the untreated HA\u20101 cells. This may be one of the mechanisms involved in the increased resistance and a common adaptive mechanism induced by both stresses. These data indicate that mammalian cells develop resistance to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> following mild pretreatment with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> or heat shock. The cross\u2010resistance induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and heat shock reinforce the hypothesis that some overlap in mechanisms exist between the cellular responses to these two stresses. However, the failure of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> pretreatment to induce much resistance to heat indicates that there are also differences in the actions of the two agents.<\/jats:p>","DOI":"10.1002\/jcp.1041310308","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:50:39Z","timestamp":1109418639000},"page":"364-373","source":"Crossref","is-referenced-by-count":180,"title":["Hydrogen peroxide or heat shock induces resistance to hydrogen peroxide in Chinese hamster fibroblasts"],"prefix":"10.1002","volume":"131","author":[{"given":"Douglas R.","family":"Spitz","sequence":"first","affiliation":[]},{"given":"W. 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