{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T09:51:52Z","timestamp":1781603512217,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6031,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1988,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myeloperoxidase (MPO) is a major heme enzyme involved in inflammatory responses of polymorphonuclear leukocytes. Using cDNA and intron specific probes for MPO we studied the regulation of MPO expression during myeloid differentiation of the promyelocytic HL\u201060 leukemia cell line. Mature MPO mRNA species of 3.3, 2.8 and 1.6 kb and heterogenous nuclear (hn)RNA of &gt; 8 and \u223c4 kb were observed in wildtype HL\u201060 cells. Induction of differentiation of the cells towards either granulocytes or macrophages resulted in a profound decrease (&gt; 95%) in the concentration of MPO mRNA levels, showing that gene expression of MPO mRNA is closely linked to the stage of development of myeloid cells. Studies using normal and leukemic hematopoietic cells confirmed these findings and showed that myeloblasts and promyelocytes contain MPO mRNA. Rate of transcription of MPO was measured by a nuclear run\u2010on assay in wild\u2010type and day 3\u2013 and day \u20134 differentiated HL\u201060 cells and was nearly the same in all three. In contrast, rate of transcription of c\u2010<jats:italic>myc<\/jats:italic> in the same nuclei became almost undetectable with induction of differentiation. Overall transcription decreased by 60% and 80% on day 3 and 4 of differentiation, respectively, compared to wild\u2010type cells. Stability of mature MPO mRNA was also measured and found to be the same in wild\u2010type and differentiated HL\u201060. Half\u2010life of MPO hnRNA was \u2264 30 min in wild\u2010type HL\u201060; nevertheless, this hnRNA was easily detectable 3 days after induction of differentiation of these cells. Taken together, the results show that decreased expression of MPO mRNA with differentiation occurs in part post\u2010transcriptionally, possibly due to a failure in RNA processing. In addition, as overall transcription decreases during differentiation, MPO transcription is concomitantly reduced. This indicates that transcriptional and post\u2010transcriptional mechanisms cooperate in the control of MPO gene expression.<\/jats:p>","DOI":"10.1002\/jcp.1041360203","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T10:34:42Z","timestamp":1109414082000},"page":"215-225","source":"Crossref","is-referenced-by-count":78,"title":["Regulation of gene expression of myeloperoxidase during myeloid differentiation"],"prefix":"10.1002","volume":"136","author":[{"given":"Andreas","family":"Tobler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl W.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith R.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Selsted","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Rovera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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