{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:42:19Z","timestamp":1770475339995,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5939,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175\u2010kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyrosine kinase activity, thought to be involved in the mitogenic signalling process. Here we have constructed a recombinant murine retrovirus that transduces a human cDNA encoding the 175\u2010kD protein and used this retrovirus to infect BAF3, a murine, bone marrow\u2010derived cell line, which is dependent on the haematopoietic factor interleukin\u20103 (IL3) for its growth in culture. The EGF receptors expressed on the infected cells exhibit two affinity states, as well as EGF\u2010stimulated autophosphorylation. Furthermore, EGF can replace IL3 in supporting short\u2010term proliferation of these cells. These data identify functional properties of the EGF receptor upon expression of the 175\u2010kD EGF binding protein in a haemotopoietic cell that does not express endogenous receptors. They also suggest that gene transfer of growth factor receptors to heterologous cells may allow novel growth stimuli to be exploited.<\/jats:p>","DOI":"10.1002\/jcp.1041370212","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T10:57:08Z","timestamp":1109415428000},"page":"293-298","source":"Crossref","is-referenced-by-count":38,"title":["Transfer of functional EGF receptors to an IL3\u2010dependent cell line"],"prefix":"10.1002","volume":"137","author":[{"given":"Mary K. L.","family":"Collins","sequence":"first","affiliation":[]},{"given":"Julian","family":"Downward","sequence":"additional","affiliation":[]},{"given":"Atsushi","family":"Miyajima","sequence":"additional","affiliation":[]},{"given":"Kazuo","family":"Maruyama","sequence":"additional","affiliation":[]},{"given":"Ken\u2010Ichi","family":"Arai","sequence":"additional","affiliation":[]},{"given":"Richard C.","family":"Mulligan","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90365-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.001205"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/328820a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041180205"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.20.6349"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80048-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6328658"},{"key":"e_1_2_1_9_1","first-page":"707","article-title":"Synthesis of mRNAs for transforming growth factors \u03b1 and \u03b2 and the epidermal growth factor receptor by human tumours","volume":"47","author":"Derynck R.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90592-7"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/315235a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90341-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.12.4568"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1311"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.23.8477"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.8.2150"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02740.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/313144a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90006-1"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"12490","DOI":"10.1016\/S0021-9258(18)67114-5","article-title":"Reconstitution of human epidermal growth factor receptors and its deletion mutants in cultured hamster cells","volume":"261","author":"Linveh E.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1002\/j.1460-2075.1987.tb02558.x","article-title":"An insertional mutant of epidermal growth factor receptor allows dissection of diverse receptor functions","volume":"6","author":"Linveh E.","year":"1987","journal-title":"EMBO J."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475-4754.1982.tb01001.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90344-6"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"12384","DOI":"10.1016\/S0021-9258(18)67251-5","article-title":"Identification of distinct receptors for two hemopoietic growth factors (granulocyte colonystimulating factor and multipotential colony\u2010stimulating factor) by chemical cross\u2010linking","volume":"261","author":"Nicola N. A.","year":"1986","journal-title":"J. Biol. 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