{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:53:47Z","timestamp":1758401627264},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5758,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1989,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The production of Chinese hamster ovary (CHO) cell mutants which are defective in endocytosis has led to a greater understanding of the process by which cells sort ligands and their receptors. Robbins and coworkers have obtained CHO mutants which are resistant to diphtheria toxin, defective in the delivery of endocytosed lysosomal enzymes to lysosomes, and have a decreased uptake of iron from transferrin (Robbins et al.: 1. Cell Biol. 96:1064\u20131071, 1983). We have previously shown that these CHO mutants are markedly deficient in the acidification of early endocytic compartments (Yamashiro and Maxfield: J. Cell Biol. 105:2713\u20132721, 1987). In this study we examined the endocytosis of \u03b1<jats:sub>2<\/jats:sub>\u2010macroglobulin (\u03b1<jats:sub>2<\/jats:sub>M) to determine whether the defects in early endosome acidification would alter the processing of this ligand. We found that the CHO mutants DTG 1\u20135\u20134 and DTF 1\u20135\u20131 bind, internalize, and degrade <jats:sup>125<\/jats:sup>l\u2010\u03b1<jats:sub>2<\/jats:sub>M in a manner similar to the wild\u2010type cells. We also found that the CHO mutants retain the ability to recycle the receptors for \u03b1<jats:sub>2<\/jats:sub>M. Since the binding of \u03b1<jats:sub>2<\/jats:sub>M is greatly reduced at mildly acidic pH (\u2248\ufe016.8), only slight acidification of the endosomal compartment should be sufficient to achieve sorting of a2M from its receptor. In contrast, lysosomal enzymes require more acidic conditions (pH &lt; 6.0) for dissociation. The different behavior of the two ligands provides biochemical evidence for a partial (but not complete) defect in early endosome acidification in the mutants. The data also indicate that pH regulation in a relatively narrow range can achieve differential sorting of various ligands.<\/jats:p>","DOI":"10.1002\/jcp.1041390221","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T10:12:39Z","timestamp":1109412759000},"page":"377-382","source":"Crossref","is-referenced-by-count":32,"title":["Kinetics of \u03b1<sub>2<\/sub>\u2010macroglobulin endocytosis and degradation in mutant and wild\u2010type Chinese hamster ovary cells"],"prefix":"10.1002","volume":"139","author":[{"given":"Darrell J.","family":"Yamashiro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence A.","family":"Borden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick R.","family":"Maxfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34768-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90340-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90052-1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.3.7.1283"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1561"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.85.3.839"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86019-2"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"3191","DOI":"10.1016\/S0021-9258(18)32848-5","article-title":"Intracellular dissociation of receptor\u2010bound asialoglycoproteins in cultured hepatocytes: A\u2010pH\u2010mediated non\u2010lysosomal event","volume":"258","author":"Harford J.","year":"1983","journal-title":"J. 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