{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:18:50Z","timestamp":1762100330139},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5240,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Growth factor\u2010depleted Swiss 3T3 cells responded to basic fibroblast growth factor (bFGF) with a burst of mitogenesis and with a rapid and marked increase in thrombospondin (TS) mRNA levels. mRNA levels for the \u03b11 chain of type I collagen and for fibronectin were unaffected. At early times following stimulation (0\u20132 h), \u201csuperinduction\u201d of TS mRNA by inhibition of protein synthesis with cycloheximide was not observed and the increase in TS mRNA could be attributed primarily to an increase in transcription rate of the TS gene. However, at later times (4\u20138 h) the combination of cycloheximide and bFGF superinduced TS mRNA levels, suggesting the existence of a labile inhibitor of transcription or a short\u2010lived RNase that might be produced in response to prolonged treatment with bFGF. In contrast to its stimulatory effect on 3T3 cells, bFGF did not stimulate the proliferation of mouse muscle BC3H1 cells nor did it cause an increase in TS mRNA levels, but BC3H1 cells do respond to bFGF by inhibition of myogenic differentiation. We propose, on the basis of these and other findings, that TS facilitates the progression of some anchorage\u2010dependent cells through the cell cycle.<\/jats:p>","DOI":"10.1002\/jcp.1041450104","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T09:13:37Z","timestamp":1109409217000},"page":"16-23","source":"Crossref","is-referenced-by-count":36,"title":["Thrombospondin gene expression is associated with mitogenesis in 3T3 cells: Induction by basic fibroblast growth factor"],"prefix":"10.1002","volume":"145","author":[{"given":"Dorit B.","family":"Donoviel","sequence":"first","affiliation":[]},{"given":"Sharon L.","family":"Amacher","sequence":"additional","affiliation":[]},{"given":"Kevin W.","family":"Judge","sequence":"additional","affiliation":[]},{"given":"Paul","family":"Bornstein","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2904"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/S0021-9258(19)77919-8","article-title":"The first intron of the \u03b11(I) collagen gene contains several transcriptional regulatory elements","volume":"263","author":"Bornstein P.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90166-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041390106"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113490"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.6.1670"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90158-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90037-5"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1016\/S0021-9258(19)35450-X","article-title":"Acidic and basic fibroblast growth factor stimulate tyrosine kinase activity in vivo","volume":"263","author":"Coughlin S. R.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.44.2.674-682.1982"},{"key":"e_1_2_1_13_1","first-page":"655","article-title":"Transient induction of c\u2010fos and c\u2010myc is an immediate consequence of growth factor stimulation","volume":"4","author":"Curran T.","year":"1985","journal-title":"Cancer Surveys"},{"key":"e_1_2_1_14_1","article-title":"The thrombospondin gene is induced by basic fibroblast growth factor (bFGF) and interleukin 1 (IL\u20101)","volume":"107","author":"Donoviel D. B.","year":"1988","journal-title":"J. Cell. Biol."},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"18590","DOI":"10.1016\/S0021-9258(18)37324-1","article-title":"Structural analysis and expression of the human thrombospondin gene promoter","volume":"263","author":"Donoviel D.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00320a049"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041330405"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/311433a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.1.3.281"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.729"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.1016\/S0021-9258(17)35666-1","article-title":"Transforming growth factorbeta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix","volume":"261","author":"Ignotz R. A.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113986"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90092-2"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00374a014"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90037-7"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"11222","DOI":"10.1016\/S0021-9258(18)60452-1","article-title":"Characterization of the promoter region of the human thrombospondin gene: DNA sequences within the first intron increase transcription","volume":"264","author":"Laherty C. D.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.6.2194"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.5.1182"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1182\/blood.V67.5.1197.1197","article-title":"The structural and functional properties of thrombospondin","volume":"67","author":"Lawler J.","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2351"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-1915-3_2"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.3.1059"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.23.9050"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.2.415"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47488-1"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"9050","DOI":"10.1016\/S0021-9258(19)86807-2","article-title":"Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct","volume":"254","author":"McKnight S.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.15.4987"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.18.7035"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.2.773"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041200304"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.3.1309"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1603"},{"key":"e_1_2_1_43_1","doi-asserted-by":"crossref","first-page":"11694","DOI":"10.1016\/S0021-9258(17)44284-0","article-title":"Biosynthesis and regulation of type V collagen in diploid human fibroblasts","volume":"258","author":"Narayanan A. S.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00360a001"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.4.1105"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90303-5"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.12.4167"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.1.131"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.341"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1003504"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.61.2.398"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90517-7"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00365a032"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90341-7"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.6.3.245"},{"key":"e_1_2_1_56_1","doi-asserted-by":"crossref","first-page":"9959","DOI":"10.1016\/S0021-9258(18)67609-4","article-title":"Tissue plasminogen activator and urokinase enhance the binding of plasminogen to thrombospondin","volume":"261","author":"Silverstein R. L.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_57_1","doi-asserted-by":"crossref","first-page":"10346","DOI":"10.1016\/S0021-9258(17)39254-2","article-title":"Activation of immobilized plasminogen by tissue activator multimolecular complex formation","volume":"260","author":"Silverstein R. L.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_58_1","doi-asserted-by":"crossref","first-page":"9483","DOI":"10.1016\/S0021-9258(18)67682-3","article-title":"Serum and fibroblast growth factor inhibit myogenic differentiation through a mechanism dependent on protein synthesis and independent of cell proliferation","volume":"261","author":"Spizz G.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_59_1","first-page":"601","article-title":"Effects of growth factors in vivo. I. Cell ingrowth into porous subcutaneous chambersI. Cell ingrowth into porous","volume":"129","author":"Sprugel K. H.","year":"1987","journal-title":"Am. J. Pathol."},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.5.1799"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.1.6.3315806"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2438772"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113486"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90178-3"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80098-9"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041450104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041450104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T14:13:35Z","timestamp":1697984015000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041450104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,10]]}},"alternative-id":["10.1002\/jcp.1041450104"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041450104","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10]]}}}