{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:15:49Z","timestamp":1779340549556,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4417,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>SPARC (secreted protein, acidic and rich in cysteine), also known as osteonectin, is an extracellular Ca<jats:sup>+2<\/jats:sup>\u2010glycoprotein that inhibits the incorporation of [<jats:sup>3<\/jats:sup>H]\u2010and delays the onset of S\u2010phase in synchronized cultures of bovine aortic endothelial (BAE) cells. This effect appears not to be dependent on the functional properties of SPARC associated with changes in cell shape or inhibition of cell spreading. In this study we investigate the conditions under which cell cycle modulation occurs in different types of cells. Human umbilical vein endothelial cells, a transformed fetal BAE cell line, and bovine capillary endothelial cells exhibited a sensitivity to SPARC and a cationic peptide from a non\u2010Ca<jats:sup>+2<\/jats:sup>\u2010region of SPARC (peptide 2.1, 0.2\u20140.8 mM) similar to that observed in BAE cells. In contrast, human foreskin fibroblasts and fetal bovine ligament fibroblasts exhibited an increase in the incorporation of [<jats:sup>3<\/jats:sup>H]\u2010in the presence of 25 \u03bcM\u20140.2 mM peptide 2.1; inhibition was observed at concentrations in excess of 0.4 mM. This biphasic modulation could be further localized to a sequence of 10 amino acids comprising the N\u2010terminal half of peptide 2.1. A synthetic peptide from another cationic region of SPARC (peptide 2.3) increased [<jats:sup>3<\/jats:sup>H]\u2010incorporation by BAE cells and fibroblasts in a dose\u2010dependent manner. In endothelial cells, a stimulation of 50% was observed at a concentration of 0.01 mM; fibroblasts required \u223c 100\u2010fold more peptide 2.3 for levels of stimulation comparable to those obtained in endothelial cells. The observation that SPARC and unique SPARC peptides can differentially influence the growth of fibroblasts and endothelial cells in a concentration\u2010dependent manner suggests that SPARC might regulate proliferation of specific cells during wound repair and remodeling. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041540108","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T07:15:55Z","timestamp":1109402155000},"page":"53-63","source":"Crossref","is-referenced-by-count":108,"title":["Differential effects of SPARC and cationic SPARC peptides on DNA synthesis by endothelial cells and fibroblasts"],"prefix":"10.1002","volume":"154","author":[{"given":"Sarah E.","family":"Funk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Helene","family":"Sage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1659742"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90448-N"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.5.2021"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.9.2919"},{"key":"e_1_2_1_6_1","first-page":"1121","article-title":"Inhibition of angiogenesis and tumor growth in the brain","volume":"137","author":"Brem S. S.","year":"1990","journal-title":"Amer. J. Pathol."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.6.3147"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6487"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb10466.x"},{"key":"e_1_2_1_10_1","first-page":"80","volume-title":"Receptors for Extracellular Matrix","author":"End P.","year":"1991"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00396a015"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(92)90471-J"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2648"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041480213"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90181-W"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.5.1419"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.9.3299"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240490310"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"13178","DOI":"10.1016\/S0021-9258(18)98821-6","article-title":"SPARC induces the expression of type I plasminogen activator inhibitor in cultured bovine aortic endothelial cells","volume":"266","author":"Hasselaar P.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.8.555"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.1.473"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00313-X"},{"key":"e_1_2_1_23_1","first-page":"174","article-title":"Differential expression of extracellular proteins is correlated with angiogenesis in vitro","volume":"64","author":"Iruela\u2010Arispe M\u2010L.","year":"1991","journal-title":"Lab. Invest."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.11.4.805"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.6.3065"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"16736","DOI":"10.1016\/S0021-9258(18)42063-7","article-title":"Regulation of gene expression by SPARC during angiogenesis in vitro: Fibronectin. thrombospondin, and type I plasminogen activator inhibitor","volume":"267","author":"Lane T. F.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650020514"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80509-X"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb17997.x"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04383.x"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.90.2.332"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.4.1127"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.5.981"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2683075"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)47121-8"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041020205"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.116.2.533"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.4.1281"},{"key":"e_1_2_1_39_1","first-page":"1183","article-title":"Ceruloplasmin, copper ions, and angiogenesis","volume":"69","author":"Raju K. S.","year":"1982","journal-title":"J. Nat. Can. Inst."},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041420307"},{"key":"e_1_2_1_41_1","doi-asserted-by":"crossref","first-page":"14831","DOI":"10.1016\/S0021-9258(18)98545-5","article-title":"Extracellular proteins that modulate cell\u2010matrix interactions: SPARC, tenascin, and thrombospondin","volume":"266","author":"Sage E. H.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.6.2723400"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.341"},{"key":"e_1_2_1_44_1","volume-title":"Proc. Fourth Int'l Conf. Chem. Biol. Mineraliz. Tiss.","author":"Sage E. H.","year":"1992"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977198909069076"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6635659"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.6.1329"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1659741"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041540108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041540108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T22:02:48Z","timestamp":1698271368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041540108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1002\/jcp.1041540108"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041540108","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}