{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:17:14Z","timestamp":1762078634011},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4144,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1993,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During wound healing, pericellular proteolysis is thought to be essential for the detachment of keratinocytes from basement membrane and in their migration into the wound bed. We have characterized integrin\u2010type cell adhesion\/migration receptors in human mucosal keratinocytes and examined their function in the regulation of type IV collagenase gene expression. Two major integrins of the \u03b21 class, \u03b12\u03b21 and \u03b1\u03b21, were found to function as collagen and fibronectin receptors, respectively. Antibodies against \u03b21 and \u03b13 integrin subunits were found to stimulate the expression of the 92 kDa type IV collagenase severalfold in a dosedependent manner. Keratinocytes expressed also the 72 kDa type IV collagenase, the synthesis of which remained, however, unchanged in keratinocytes treated with anti\u2010integrin antibodies. Stimulation of 92 kDa enzyme was found to be caused directly by antibody binding to integrins, since Fab\u2010fragments of anti\u2010\u03b21 antibodies alone were able to induce collagenase expression in the absence of secondary, clustering antibodies. Antibodies against \u03b12\u03b21 integrin caused no stimulation. Keratinocytes seeded on different substrata (plastic, collagen, fibronectin, laminin, or vitronectin) showed equal induction of type IV collagenase expression. Expression of 92 kDa type IV collagenase could not be induced by peptides (GRGDS, GRGES), proteins (fibronectin, laminin, fibrinogen., albumin), or antibodies to fibronectin. We suggest that proteolytic processes around keratinocytes can be regulated by extracellular factors signalling through integrin\u2010type receptors. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041570125","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T05:35:20Z","timestamp":1109396120000},"page":"190-200","source":"Crossref","is-referenced-by-count":65,"title":["Anti\u2010integrin antibodies induce type IV collagenase expression in keratinocytes"],"prefix":"10.1002","volume":"157","author":[{"given":"Hannu","family":"Larjava","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Guy","family":"Lyons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marja","family":"M\u00e4kel\u00e4","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leeni","family":"Koivisto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Birkedal\u2010Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven K.","family":"Akiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth M.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyrki","family":"Heino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90175-E"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.3.829"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"17536","DOI":"10.1016\/S0021-9258(18)45414-2","article-title":"Biosynthesis and acquisition of biological activity of the fibronectin receptor","volume":"262","author":"Akiyama S. K.","year":"1987","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.863"},{"key":"e_1_2_1_6_1","first-page":"1601","article-title":"Differences in the biosynthesis and localization of the fibronectin receptor in normal and transformed cultured human cells","volume":"50","author":"Akiyama S. K.","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.11.2199285"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(82)90388-5"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90268-1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.4.1387"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.6.3141"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90092-D"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.5.1649"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68680-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.1.183"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.17.6888"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.1.169"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.7.2234"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42768-2"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3940-7_20"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42873-6"},{"key":"e_1_2_1_22_1","first-page":"308","article-title":"A murine monoclonal antibody that identifies a 157\/130 kDa platelet\u2010collagen receptor","volume":"78","author":"Gralnick H. R.","year":"1989","journal-title":"Circulation"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.2.11.951"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31269-3"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1242\/dev.112.1.193","article-title":"Integrin expression during human epidermal development in vivoard in vitro","volume":"112","author":"Hertle M. D.","year":"1991","journal-title":"Development"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90338-3"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/320531a0"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90115-S"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.6.1249"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03425.x"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.6.2279"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90807-Q"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00510080"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.19.8392"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90710-0"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.2.437"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199101000-00012"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.3.803"},{"key":"e_1_2_1_39_1","first-page":"266","article-title":"Immunolocalization of beta 1 integrins in human gingival epithelium and cultured keratinocytes","volume":"100","author":"Larjava H.","year":"1993","journal-title":"Scand. J. Dent. Res."},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.3.671"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.3.249"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45827-9"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.4.761"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(90)90126-Q"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90009-2"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(82)90272-9"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2580463"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0180812"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2770277"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90199-4"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19462.x"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.2.721"},{"key":"e_1_2_1_53_1","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1016\/S0021-9258(18)94267-5","article-title":"Independent regulation of collagenase. 72\u2010kDa progelatinase, and metalloendoproteinase inhibitor expression in human fibroblasts by transforming growth factor\u2010\u03b2","volume":"264","author":"Overall C. M.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)92810-3"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114379"},{"key":"e_1_2_1_56_1","doi-asserted-by":"crossref","first-page":"4568","DOI":"10.1016\/S0021-9258(18)68822-2","article-title":"Transforming growth factor\u2010\u03b2 stimulates the expression of fibronectin and of both subunits of the human fibronectin receptor by cultured human lung fibroblasts","volume":"263","author":"Roberts C. J.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114957"},{"key":"e_1_2_1_58_1","doi-asserted-by":"crossref","first-page":"3058","DOI":"10.1016\/S0021-9258(18)32829-1","article-title":"Purification and characterization of murine basement membrane collagen\u2010degrading enzyme secreted by metastatic tumor cells","volume":"258","author":"Salo T.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_59_1","doi-asserted-by":"crossref","first-page":"11436","DOI":"10.1016\/S0021-9258(18)98977-5","article-title":"Transforming growth factor\u2010\u03b21 up\u2010regulates type IV collagenase expression in cultured human keratinocytes","volume":"266","author":"Salo T.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.12.4525"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.17.7849"},{"key":"e_1_2_1_62_1","doi-asserted-by":"crossref","first-page":"3822","DOI":"10.1016\/S0021-9258(19)84924-4","article-title":"Cleavage of type VII collagen by interstitial collagenase and type IV collagenase (gelatinase) derived from human skin","volume":"264","author":"Seltzer J. L.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)30519-7"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)89530-1"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.22.8970"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00049520"},{"key":"e_1_2_1_67_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.661"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.3.1059"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1873"},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77378-X"},{"key":"e_1_2_1_71_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.3.697"},{"key":"e_1_2_1_72_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.877"},{"key":"e_1_2_1_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71480-4"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041570125","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.1041570125","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.1041570125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T05:45:25Z","timestamp":1698299125000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.1041570125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10]]},"references-count":72,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,10]]}},"alternative-id":["10.1002\/jcp.1041570125"],"URL":"https:\/\/doi.org\/10.1002\/jcp.1041570125","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10]]}}}