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The proliferation of the various cell lines was suppressed by adding bafilomycin A<jats:sub>1<\/jats:sub> to the culture medium. This inhibitory effect appeared at a concentration of nanomolar order and was dose dependent. Although the suppression was reversible, the drug exerted not only suppression of the proliferation but also death to some cell lines. Drug concentration required for 50% inhibition of the cell proliferation during 48 h differed markedly depending on cell species and the sensitivity appears to increase by the transformation of the cells. Two derivatives of concanamycin A, an analogue of bafilomycin A<jats:sub>1<\/jats:sub>, also inhibited strongly V\u2010AT\u2010Pase in vitro and in vivo, and simultaneously cell proliferation. Two concanamycin A derivatives which have lost inhibitory effect on V\u2010ATPase lost inhibitory effect on cell proliferation as well. These results suggest that V\u2010ATPase is involved in the machinery maintaining the cell proliferation. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041570303","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T05:16:58Z","timestamp":1109395018000},"page":"445-452","source":"Crossref","is-referenced-by-count":58,"title":["Inhibitors of vacuolar\u2010type H<sup>+<\/sup>\u2010ATPase suppresses proliferation of cultured cells"],"prefix":"10.1002","volume":"157","author":[{"given":"Tsukasa","family":"Manabe","sequence":"first","affiliation":[]},{"given":"Tamotsu","family":"Yoshimori","sequence":"additional","affiliation":[]},{"given":"Nobuhiro","family":"Henomatsu","sequence":"additional","affiliation":[]},{"given":"Yutaka","family":"Tashiro","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"C586","DOI":"10.1152\/ajpcell.1990.259.4.C586","article-title":"ATP\u2010dependent pHi recovery in lung macrophages: Evidence for a plasma membrane H+\u2010ATPase","volume":"259","author":"Bidani A.","year":"1990","journal-title":"Am. 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