{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:28:08Z","timestamp":1759937288421},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":3506,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[1995,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We isolated two cell clones from the wild\u2010type Madin\u2010Darby canine kidney cell line (MDCK) that resembles renal collecting duct epithelium. Morphology and karyotypes of the two cell clones were evaluated. The MDCK\u2010C7 cell clone morphologically resembles principal cells (polygonal cell shape, flat), while the MDCK\u2010C11 clone resembles intercalated cells (cuboidal cell shape, high). The diploid chromosome number of MDCK\u2010C7 cells is 83.1 \u00b1 0.2 (n = 139); that for MDCK\u2010C11 cells is 78.8 \u00b1 0.1 (n = 128). Culture of MDCK\u2010C7 cells in alkaline medium (pH 7.7) induced irreversible phenotypical and genotypical alterations. transformed MDCK\u2010C7F cells are characterized by two abnormal (biarmed) chromosomes. In contrast, MDCK\u2010C11 cells are not phenotypically altered by alkaline stress. In order to elucidate the role of intracellular pH (pH<jats:sub>i<\/jats:sub>) in the transformation process, we measured pH<jats:sub>i<\/jats:sub> under control conditions (pH 7.4). after 5 min exposure to alkaline stress (\u201cacute experiment,\u201d pH 7.7) and after incubation of the cells in alkaline medium for two weeks (\u201cchronic experiment,\u201d pH 7.7). Under control conditions, MDCK\u2010C7 cells maintained pH<jats:sub>i<\/jats:sub> at 7.14 \u00b1 0.01 (n = 154) and MDCK\u2010C11 cells at 7.01 \u00b1 0.01 (n = 147). Acute alkaline stress increased pH<jats:sub>i<\/jats:sub> of both cell types to similar steady\u2010state values. Under chronic alkaline stress, MDCK\u2010C7 cells were unable to maintain intracellular pH within normal limits exhibiting sustained alkalinization, whereas MDCK\u2010C11 cells could successfully regulate pH<jats:sub>i<\/jats:sub>. We conclude that wild\u2010type MDCK cells consist of two genetically distinct subpopulations with different morphology and function. Only the MDCK\u2010C7 clone that resembles the principle cell type of renal collecting duct can be transformed by alkaline stress while the MDCK\u2010C11 clone resists this treatment, due to efficient pH<jats:sub>i<\/jats:sub> control mechanisms. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.1041640121","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T04:32:38Z","timestamp":1109392358000},"page":"164-171","source":"Crossref","is-referenced-by-count":39,"title":["Phenotypically and karyotypically distinct Madin\u2010Darby canine kidney cell clones respond differently to alkaline stress"],"prefix":"10.1002","volume":"164","author":[{"given":"S.","family":"W\u00fcnsch","sequence":"first","affiliation":[]},{"given":"M.","family":"Gekle","sequence":"additional","affiliation":[]},{"given":"U.","family":"Kersting","sequence":"additional","affiliation":[]},{"given":"B.","family":"Schuricht","sequence":"additional","affiliation":[]},{"given":"H.","family":"Oberleithner","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-122-31293"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374853"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000154638"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/313481a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869207"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000154667"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1016\/S0021-9258(18)61222-0","article-title":"Na+\u2010independent Cl\u2212\/HCO3\n                  \u2212 exchange in Madin\u2010Darby canine kidney cells","volume":"262","author":"Kurtz I.","year":"1987","journal-title":"J. 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