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Application of a series of lectins of different sugar specificities permits a differentiation between Golgi subcompartments containing glycoconjugates with different oligosaccharide side chains. These may be (a) different glycoconjugates or (b) glycoconjugates at different stages during synthesis or repair of their glycans. The lectinocytochemical studies with mannose\u2010, glucose\u2010, N\u2010acetyl\u2010glucosamine\u2010, N\u2010acetylgalactosamine\u2010, galactose\u2010, fucose\u2010, and sialic acid\u2010recognizing lectins revealed predominating patterns that labeled distinct, i.e., cis, medial, trans, and transmost, regions of the Golgi apparatus. A further refinement could be achieved by differential lectin\u2010inhibition that enables a dissection of lectin binding reactions on the basis of their binding affinities. High\u2010affinity binding reactions showed that subcompartments are not necessarily confined to one single Golgi subregion and may change their position from one to another subregion. Some of the patterns observed may be interpreted in relation to certain steps during synthesis and modifications of glycans.<\/jats:p>","DOI":"10.1002\/jemt.1060170106","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T08:39:06Z","timestamp":1109234346000},"page":"35-50","source":"Crossref","is-referenced-by-count":12,"title":["Cytochemical characteristics of the golgi apparatus"],"prefix":"10.1002","volume":"17","author":[{"given":"Margit","family":"Pavelka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolf","family":"Ellinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"102","volume-title":"Cell BIology of the Secretory Process","author":"Bennett G.","year":"1984"},{"key":"e_1_2_1_3_1","first-page":"111","article-title":"The functional organization of the Golgi apparatus","volume":"126","author":"Berger E. 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