{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T14:19:19Z","timestamp":1699971559188},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":6980,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1986,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hepatitis B virus (HBV) genome carries an open reading frame of 462 bases, the X region, but the corresponding protein has yet to be identified as a natural product. In rodent cells cotransformed with the thymidine kinase gene of herpes simplex virus and HBV DNA, however, Gough [1983] identified a mRNA that hybridises uniquely with the X region of the HBV genome. A large fragment of the X region was inserted into plasmid pCL19LDY\u2010T in order to produce, in <jats:italic>Escherichia coli<\/jats:italic>, the X gene product, HBxAg, as a polypeptide fused to the N\u2010terminal part of the phage \u00fecro gene product. Antisera raised against this fused polypeptide gave positive immunofluorescence reactions with the transformed rodent cells. This provides direct evidence for the expression of the HBxAg gene in eukaryotic cells transformed with HBV DNA. The approach used here should be generally applicable.<\/jats:p>","DOI":"10.1002\/jmv.1890200305","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T12:51:30Z","timestamp":1172839890000},"page":"229-246","source":"Crossref","is-referenced-by-count":30,"title":["Expression of the X Gene of Hepatitis B Virus"],"prefix":"10.1002","volume":"20","author":[{"given":"John 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