{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:00:08Z","timestamp":1770750008960,"version":"3.50.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":6858,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Region 65\u2010102 of the myelin basic protein (MBP) houses a number of antigenic determinants known to induce delayed\u2010type hypersensitivity, experimental allergic encephalomyelitis (EAE), suppressor cell function, and antibodies. In this report we describe the biological activity of synthetic peptides S53, S55, and S49 with sequence homology to region 69\u201084 of the rat, guinea pig, and bovine MBP, Peptide S53\u2010A, defined by residues 75\u201084 of the guinea pig (SQRSQDEN) and of the rat (SQRTQDEN) MBP induced clinical signs of disease in Lewis rats. These included weight loss, flaccid tail, \u201cmuscle wasting\u201d and hind\u2010leg weakness. Histological examination of brain, spinal cord, and sciatic nerve sections of diseased rats revealed the complete absence of focal and perivascular lymphocytic infiltrates characteristic of demyelinating EAE lesions. Elongation of peptide S53 by three or six residues to residue sequences naturally found at its N\u2010terminal end gave rise to peptides S55S (PQKSQRSQDEN) and S49S (GSLPQKSQRSDQDEN), respectively. Lewis rats challenged with either S55S or S49S developed classical clinical and histological signs of EAE. Severe hindleg paralysis was accompanied by incontinence and sometimes death. Injected in the form of carrier\u2010free peptide, S53 was a meager B cell immunogen. S53 conjugated with methylated\u2010bovine serum albumin was also a potent immunogen and produced clinical signs of disease without CNS pathology. By comparison, carrier\u2010free S55S and S49S were potent immunogens giving rise to antibodies that cross reacted completely and competitively with S55S but considerably less so with S53. The results show that the sequence of S53 defines an epitope responsible for the formation of anti\u2010S53 antibodies. Elongation of the S53 sequence at its N\u2010terminal end generated an additional epitope which induced cell\u2010mediated immunity responsible for the concomitant development of pathological signs of EAE. It may be concluded that the induction of classical signs of EAE requires specific and defined sequences capable of expressing both B cell and T cell functions.<\/jats:p>","DOI":"10.1002\/jnr.490160303","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:27:05Z","timestamp":1104542825000},"page":"467-478","source":"Crossref","is-referenced-by-count":51,"title":["Biological activity of region 65\u2013102 of the myelin basic protein"],"prefix":"10.1002","volume":"16","author":[{"given":"G.A.","family":"Hashim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.D.","family":"Day","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Fredane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Intintola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1330529"},{"key":"e_1_2_1_3_1","first-page":"313","volume-title":"Phsiology and Pathology of Axons","author":"Bornstein MB","year":"1978"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb20212.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.148.3674.1242"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90399-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-3917-5_1"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00988601"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(81)90045-X"},{"key":"e_1_2_1_10_1","first-page":"1","article-title":"Monoclonal and polyclonal antibodies to myelin basic protein determinants","volume":"10","author":"Day ED","year":"1986","journal-title":"J Neuroimmunol"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"5770","DOI":"10.1016\/S0021-9258(18)61872-1","article-title":"Basic Al protein of the myelin membrane. The complete amino acid sequence","volume":"246","author":"Eylar EH","year":"1971","journal-title":"J Biol Chem"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(82)90484-1"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1016\/S0021-9258(18)62319-1","article-title":"Glycosylation of the Al protein form myelin by a polypeptide N\u2010acetylgalactosaminyltransferase: Identification of the receptor sequence","volume":"246","author":"Hagopian A","year":"1971","journal-title":"J Biol Chem"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1978.tb00397.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.4049\/jimmunol.126.2.419","article-title":"Experimental allergic encephalomyelitis: Activation of suppressor T lymphocytes by a modified sequence of the T effector determinant","volume":"126","author":"Hashim GA","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(74)90220-1"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1979.tb04511.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"862","DOI":"10.4049\/jimmunol.127.3.862","article-title":"Experimental allergic encephalomyelitis in Lewis rats: Immunolregulation of disease by a single amino acids substitution in the disease\u2010inducing determinant","volume":"127","author":"Kardys E","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.4049\/jimmunol.131.1.275","article-title":"A rabbit B cell determinant for a conserved protion of myelin basic protein rabit encephalitogenic sequence 65\u201074","volume":"131","author":"Lazarus KJ","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/S0021-9258(18)33140-5","article-title":"Identification of multiple in vivo phosphorylation site in rabbit myelin basic protein","volume":"258","author":"Martenson RE","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00897a025"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"516","DOI":"10.4049\/jimmunol.136.2.516","article-title":"Identification of an antigenic determinant within the phylogenetically conserved triprolyl region of myelin basic protein","volume":"136","author":"Potter NT","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.173.3998.736"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.2.317"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490160303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490160303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T18:51:46Z","timestamp":1697914306000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490160303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/jnr.490160303"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490160303","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}