{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T12:53:24Z","timestamp":1770987204707,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":6493,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A full\u2010length clone for the human proteolipid protein (PLP) was isolated from a cDNA library constructed from poly (A)<jats:sup>+<\/jats:sup> RNA isolated from fetal spinal cords obtained at 15\u201324 weeks of conceptional age. The sequence of the human PLP cDNa was determined, and the deduced amino acid sequence was found to be identical with that of rat PLP. Comparison of human and rat PLP cDNA clones indicated that the coding regions retaind 97% homology and that there were also other areas of conserved sequence. The human 5\u2032\u2010untranslated region was 93% homologous to that of the rat. The 3\u2032\u2010untranslated region was, overall, 73% homologous to that of the rat with areas containing &gt;84% homology in the first 400 and last 200 nucleotides. The most variability within the 3\u2032\u2010untranslated region occurred between nucleotides 2,000\u20132,500, where homology with the rat cDNA dropped to 55%. Expression of PLP in the human spinal cord between 11 and 23 weeks after conception was examined and compared with the expression of the myelin basic protein (MBP). RNA was isolated from pooled human spinal cords obtained at three periods of development: 11\u201314 weeks, 17\u201319 weeks, and 21\u201323 weeks. Northern blot analysis revealed a 3.2\u2010kilobase (kb) PLP mRNA that was present at higher abundance in the 21\u201323\u2010week samples. The 17\u201319\u2010week RNA than in the 17\u201319\u2010week or the 11\u201014\u2010week samples. The 17\u201019\u2013week RNA sample also contained a PLP\u2010hybridizing band at 2.2 kb which may possibly have arisen by utilization of alternative polysdenylation signals. Messenger RNA for MBP was detectable at 11\u201314 weeks but was readily evident in both the 17\u201319\u2010 and 21\u201323\u2010week age groups. Immunoblot analysis of whole spinal cord homogenates indicated that polypeptides for MBP preceded the appearance polypeptides for PLP by 3\u20134 weeks.<\/jats:p>","DOI":"10.1002\/jnr.490180303","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:33:20Z","timestamp":1104543200000},"page":"395-401","source":"Crossref","is-referenced-by-count":53,"title":["Expression of myelin proteins in the developing human spinal cord: Cloning and sequencing of human proteolipid protein cDNA"],"prefix":"10.1002","volume":"18","author":[{"given":"K. E.","family":"Kronquist","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. F.","family":"Crandall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. B.","family":"Macklin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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