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The activity of CMP\u2010NANA: ganglioside sialosyltransferase using endogenous acceptors was 0.84 pmoles NANA transferred\/mg synaptosomal protein\/hr. Analysis of the distribution of labeled sialic acid revealed that GD3 ganglioside (\u03b12\u21928 disialosyl, \u03b12\u21923 galactosyl, \u03b21\u21924 glucosyl, \u03b21\u21921\u2010 ceramide) was the major product in the membrane carrying 32% of the total lipid bound label. Treatment of the reaction products with <jats:italic>Clostridium<\/jats:italic> neuraminidase liberated labeled sialic acid from GD3 and yielded labeled GM3, then unlabeled lactosyl ceramide. Lac\u2010cer and GM3 are present in small amounts in synaptosomes, and GD3 repressents less than 2% of the total ganglioside. Our findings indicate that the sialosyltransferse activity of synaptosomes exhibits a preferential product specificity for the small pool of synaptosomal membrane GD3 ganglioside that may be formed in situ, via sialosylation of its precursor (GM3 or lactosyl ceramide) which pre\u2010exists in the synaptosomal plasma membrane. The second major labeleld product quantitatively was GD1a whose precursor substrate, GM1, is quite abundant in the membrane, so that the conversion rat of GM1 to GD1a was low in comaprison with GD3 formation. Sialosylation of other synaptosomal membrane gangliosides was negligible.<\/jats:p>","DOI":"10.1002\/jnr.490180312","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T01:33:20Z","timestamp":1104543200000},"page":"456-465","source":"Crossref","is-referenced-by-count":11,"title":["Glycolipid sialosyltransferase activity in synaptosomes exhibits a product specificity for (2\u20138)disialosy lactosyl ceramide (ganglioside GD3)"],"prefix":"10.1002","volume":"18","author":[{"given":"R.","family":"Durrie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rosenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490120208"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1973.tb00075.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1986.tb00737.x"},{"key":"e_1_2_1_5_1","first-page":"3","volume-title":"Neurochemistry of Aminosugars","author":"Brunngraber EG","year":"1979"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"7453","DOI":"10.1016\/S0021-9258(17)42812-2","article-title":"A monoclonal antibody recognizes an O\u2010acylated sialic acid in a human melanoma\u2010associated ganglioside","volume":"259","author":"Cheresh DA","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02263573"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S0021-9258(19)41958-3","article-title":"The sialic acids. 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