{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:54:41Z","timestamp":1773662081533,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":5093,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1990,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biological effects of nerve growth factor (NGF) have been shown to be mediated by the high\u2010affinity form of the nerve growth factor receptor (NGF\u2010R) in sympathetic and sensory neurons, and in PC12 cells. We report here that the central nervous system C6 rat glioma cell line likewise expresses functional high\u2010affinity NGF\u2010Rs. The expression of NGF\u2010R mRNA in C6 cells can be up\u2010regulated by cycloheximide and its own ligand, NGF; and it can be rapidly down\u2010regulated by epidermal growth factor (EGF). Furthermore, C6 cells display NGF responsiveness by expressing c\u2010fos mRNA within 30 minutes of treatment with NGF; and after 4\u20145 days of NGF exposure, C6 cells cease dividing as measured by [<jats:sup>3<\/jats:sup>H]\u2010thymidine uptake, change shape, and reveal neurite\u2010like \u2010processes. Scatchard analysis of [<jats:sup>125<\/jats:sup> I]\u2010labelled bound to solubilized C6 cells confirms the presence of both high\u2010 and low\u2010affinity receptor protein. Cross\u2010linking radiolabeled NGF to its receptor in the presence or absence of excess unlabeled NGF, followed by immunoprecipitation with monoclonal antibody (mAb) 192\u2010IgG (a known anti\u2010NGF\u2010R antibody) and SDS\u2010PAGE reveals a 100 kD band corresponding to the NGF\/NGF\u2010R complex. An identical band is observed when the immunoprecipitation is carried out with mAb 217c, suggesting that the 217c epitope is related to NGF\u2010R. The 217c antibody was generated against C6 cells and shown to be a cell surface antibody (Peng et al., Science 215:1102\u20134, 1982); several investigators have used it subsequently as an immunocytochemical marker for Schwann cells. The significance of NGF\u2010Rs in a CNS glial cell line is unclear, but association of NGF with the control of proliferation and\/or differentiation of primitive glial cells is suggested.<\/jats:p>","DOI":"10.1002\/jnr.490270320","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T05:42:37Z","timestamp":1104558157000},"page":"408-417","source":"Crossref","is-referenced-by-count":60,"title":["Characterization of functional nerve growth factor\u2010receptors in a CNS glial cell line: Monoclonal antibody 217c recognizes the nerve growth factor\u2010receptor on C6 glioma cells"],"prefix":"10.1002","volume":"27","author":[{"given":"S.","family":"Kumar","sequence":"first","affiliation":[]},{"given":"J.","family":"Huber","sequence":"additional","affiliation":[]},{"given":"L. A.","family":"Pe\u00f1a","sequence":"additional","affiliation":[]},{"given":"J. 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