{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T19:05:43Z","timestamp":1778699143972,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4789,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Neuroscience Research"],"published-print":{"date-parts":[[1991,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To better understand the function(s) of the multiphosphorylation repeat (MPR) of the high molecular weight neurofilament (NF) subunit (NF\u2010H), we sought to determine how phosphorylation within this region is regulated in cultured rat sympathetic neurons. To do this, monoclonal antibodies specific to phosphorylated or nonphosphorylated tandem repeats of the ammo acid sequence Lys\u2010Ser\u2010Pro\u2010Ala\u2010Glu\u2010Ala found within the MPR were identified and used to determined the extent of phosphorylation, the time course of phosphorylation, and the rate of turnover of phosphate groups within the NF\u2010H MPR in cultured sympathetic neurons. We showed that (1) the synthesis and phosphorylation of NF\u2010H occurs in these neurons cultured for 1 or 4 weeks; (2) the conversion from poorly to more highly phosphorylated variants of NF\u2010H occurs slowly in cultured neurons; and (3) the turnover of phosphate groups on both poorly and highly phosphorylated variants of NF\u2010H occurs more rapidly than the turnover of NF\u2010H itself. In addition, we showed that the 200\u2010kD highly phosphorylated NF\u2010H can contain at least five consecutive nonphosphorylated tandem repeats as well as phosphorylated tandem repeats, whereas we were unable to detect consecutively phosphorylated tandem repeats in the 160\u2010kD form of NF\u2010H.<\/jats:p><jats:p>These findings allow us to propose a mechanism whereby NF\u2010H is initially phosphorylated singly at sites distributed throughout the MPR. This \u201cpoorly phosphorylated NF\u2010H,\u201d which is the predominant form in immature neurons, is then converted by phosphorylation at additional sites within the MPR to the more \u201chighly phosphorylated\u201d species of NF\u2010H typically found in mature neurons. These latter phosphorylation events generate NF\u2010H variants in which tandem repeats within the MPR have been phosphorylated.<\/jats:p>","DOI":"10.1002\/jnr.490300113","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T07:16:13Z","timestamp":1104563773000},"page":"116-123","source":"Crossref","is-referenced-by-count":19,"title":["Dynamics of mammalian high\u2010molecular\u2010weight neurofilament subunit phosphorylation in cultured rat sympathetic neurons"],"prefix":"10.1002","volume":"30","author":[{"given":"E. A.","family":"Clark","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. M.\u2010Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","unstructured":"BalinBJ ClarkEA TrojanowskiJQ LeeVM\u2010Y(1991): Reassembly of bovine neurofilaments in vitro: Biochemical ultrastructural and immunoelectron microscopic studies employing mono clonal antibodies to defined epitopes.Brain Res(in press).","DOI":"10.1016\/0006-8993(91)90306-G"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-09-03296.1988"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-04-01004.1986"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"9805","DOI":"10.1016\/S0021-9258(17)39307-9","article-title":"The structure, bio chemical properties, and immunogenicity of neurofilament peripheral regions are determined by phosphorylation state","volume":"260","author":"Carden MJ","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-11-03489.1987"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","unstructured":"ClarkEA LeeVM\u2010Y(1991): The differential role of protein kinase C isozymes in the rapid induction of neurofilament phosphorylation by nerve growth factor and phorbol esters in PC 12 cells.J Neurochem(in press).","DOI":"10.1111\/j.1471-4159.1991.tb08222.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90254-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","unstructured":"De WaeghSM.LeeVM\u2010Y BradyST(1991): Modulation of neurofilament phosphorylation axonal caliber and slow axonal transport by myelinating Schwann cells. Submitted.","DOI":"10.1016\/0092-8674(92)90183-D"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2520655"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1983.tb01584.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.4.1523"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-03-00959.1989"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.10.3472"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"4019","DOI":"10.1016\/S0021-9258(18)32770-4","article-title":"The distribution of phosphorylation sites among identified proteolytic fragments of mammalian neurofilaments","volume":"258","author":"Mien J\u2010P","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1983.048.01.076"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-11-03039.1985"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"3474","DOI":"10.1523\/JNEUROSCI.07-11-03474.1987","article-title":"Monoclonal antibodies distinguish several differently phosphorylated states of the two largest rat neurofilament subunits (NF\u2010H and NF\u2010M) and demonstrate their existence in the normal nervous system of adult rats","volume":"7","author":"Lee VM\u2010Y","year":"1987","journal-title":"J Neurosci"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7384"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.5.1529"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2689"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.7.2463"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/S0021-9258(19)75826-8","article-title":"Nerve growth factor enhances the synthesis, phosphorylation, and metabolic stability of neurofilament proteins in PC 12 cells","volume":"262","author":"Lindenbaum MH","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_24_1","first-page":"2590","article-title":"Complete amino acid sequence and in vitro expression of rat NF\u2010M, the middle molecular weight neurofilament protein","volume":"7","author":"Napolitano EW","year":"1987","journal-title":"J Neurosci"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"16298","DOI":"10.1016\/S0021-9258(18)66564-0","article-title":"Differential turnover of phosphate groups on neurofilament subunits in mammalian neurons in vivo","volume":"261","author":"Nixon RA","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_26_1","first-page":"2510","article-title":"Characterization of posttranslational processing of the mammalian high\u2010molecular\u2010weight neurofilament protein in vivo","volume":"7","author":"Ohlinger MM","year":"1987","journal-title":"J Neurosci"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1523\/JNEUROSCI.09-02-00698.1989","article-title":"Lamprey neurofilaments combine in one subunit the features of each mammalian NF triplet protein but are highly phosphorylated only in large axons","volume":"9","author":"Pleasure SJ","year":"1989","journal-title":"J Neurosci"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.bi.57.070188.003113","article-title":"Molecular biology of intermediate filaments","volume":"57","author":"Steinert PM","year":"1988","journal-title":"Ann Rev Biochem"}],"container-title":["Journal of Neuroscience Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjnr.490300113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jnr.490300113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:43:12Z","timestamp":1698021792000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jnr.490300113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,9]]}},"alternative-id":["10.1002\/jnr.490300113"],"URL":"https:\/\/doi.org\/10.1002\/jnr.490300113","archive":["Portico"],"relation":{},"ISSN":["0360-4012","1097-4547"],"issn-type":[{"value":"0360-4012","type":"print"},{"value":"1097-4547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9]]}}}