{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:43:26Z","timestamp":1761155006852},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":3884,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is known that transfer of the wild\u2010type <jats:italic>p53<\/jats:italic> gene into <jats:italic>p53<\/jats:italic>\u2010negative cells from transgenic mice increases their sensitivity to drug and radiation\u2010induced apoptosis. However, unlike many human tumors, these transgenic cells do not express mutant p53, and it is not known from these earlier studies whether wild\u2010type p53 dominates the effects of mutant p53 with respect to drug and radiation sensitivity. We addressed this question in glioblastoma, a disease characterized by an unusually high level of intrinsic resistance to therapy and poor prognosis: mean survival time from diagnosis is only about 1 yr. We introduced the gene for wild\u2010type <jats:italic>p53<\/jats:italic> into human T98G glioblastoma cells, which express endogenous mutant p53 but not wild\u2010type p53. Stable transfectants that co\u2010expressed mutant and wild\u2010type p53 had enhanced sensitivity to cisplatin and gamma radiation, compared with parental cells, control vector\u2010transduced cells, and transduced cells that had lost expression of wild\u2010type p53. Transient wild\u2010type p53 expression after high\u2010efficiency gene transfer by a p53 adenovirus also sensitized the cells to cisplatin and correlated with the induction of apoptosis. The sensitization effect was also observed in p53 adenovirus\u2010infected H23 small cell lung carcinoma cells, which express endogenous mutant p53. Therefore, wild\u2010type <jats:italic>p53<\/jats:italic> gene transfer has dominant effects over mutant <jats:italic>p53<\/jats:italic> in sensitizing tumor cells to therapy, which supports the potential of <jats:italic>p53<\/jats:italic> gene therapy to enhance the efficacy of traditional therapy. \u00a9 1995 Wiley\u2010 Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.2940140408","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T21:11:30Z","timestamp":1172092290000},"page":"275-285","source":"Crossref","is-referenced-by-count":68,"title":["Use of wild\u2010type <i>p53<\/i> to achieve complete treatment sensitization of tumor cells expressing endogenous mutant p53"],"prefix":"10.1002","volume":"14","author":[{"given":"Ruth A.","family":"Gjerset","sequence":"first","affiliation":[]},{"given":"Sally T.","family":"Turla","sequence":"additional","affiliation":[]},{"given":"Robert E.","family":"Sobol","sequence":"additional","affiliation":[]},{"given":"Jason 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