{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:11:12Z","timestamp":1698225072030},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":4636,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1992,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two neuropeptide precursor cDNAs (LUQ\u20101 and L5\u201067) have been recently isolated from the Left Upper Quadrant (LUQ) neurons of the abdominal ganglion of <jats:italic>Aplysia californica<\/jats:italic> (Shyamala, Fisher, and Scheller, 1986; Wickham and DesGroseillers, 1991). Using <jats:italic>in situ<\/jats:italic> hybridization techniques as well as dot blot and polymerase chain reaction (PCR) assays, we have studied the expression of these genes in the central nervous system (CNS) of <jats:italic>Aplysia californica<\/jats:italic>. The LUQ\u20101 gene was found to be expressed in neuron L5 in the abdominal ganglion, whereas the expression of the L5\u201067 gene was observed in the other four LUQ cells (L2\u20104 and L6). When <jats:italic>in situ<\/jats:italic> hybridization was performed on paraffin sections of the abdominal ganglion, clusters of smaller cells located in the left hemiganglion, were also found to express either the LUQ\u20101 on the L5\u201067 gene, never both. In many sections, the mRNAs coding for the two neuropeptides were found not only in cell bodies but also in the axon of individual LUQ neurons and even as far as the pericardial nerve. The presence of neuropeptide mRNA in axons, pericardial nerve, and kidney has been confirmed by polymerase chain reaction. A specific, although diffuse hybridization in the left upper quadrant also suggests that mRNA is present in the neuritic field. Taken together these results indicate that neuron L5 is the only giant neuron expressing the LUQ\u20101 gene and might therefore have a physiological function different from the other four LUQ cells. Neuropeptide mRNAs were also found in the axon and\/or the neuritic field of giant neurons and could play important roles related to cell signalling in axons and nerve termini.<\/jats:p>","DOI":"10.1002\/neu.480230109","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T21:31:37Z","timestamp":1117229497000},"page":"89-101","source":"Crossref","is-referenced-by-count":15,"title":["Differential expression of neuropeptide gene mRNA within the LUQ cells of <i>Aplysia californica<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Claire","family":"Landry","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Crine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc","family":"Desgroseillers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.04-08-01944.1984"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90046-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04181.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.10.3788"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90133-X"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4179(85)90113-1"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90340-L"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840700"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1987.tb00950.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90162-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/345718a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/330477a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-09-03056.1990"},{"key":"e_1_2_1_15_1","first-page":"2762","article-title":"Aplysia californica neurons express microinjected neuropeptide genes","volume":"7","author":"DesGroseillers L.","year":"1987","journal-title":"Molec. 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