{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T19:59:53Z","timestamp":1761163193211,"version":"build-2065373602"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"vor","delay-in-days":4609,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["pathsocjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Pathology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In Hodgkin's disease, Epstein\u2013Barr virus (EBV) is found in CD30\u2010positive Reed\u2013Sternberg cells. We therefore studied 60 CD30\u2010positive non\u2010Hodgkin's lymphomas (NHLs) for the presence of EBV by the polymerase chain reaction (PCR) and DNA\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization (DISH), and by immunohistochemistry for the latent EBV proteins LMP and EBNA\u20102. CD30\u2010negative NHLs and reactive lymph nodes served as controls. The CD30\u2010positive cases comprised 17 anaplastic large cell lymphomas (ALCLs) (&gt; 75 per cent CD30\u2010positive cells) and 43 non\u2010ALCLs (with 5\u201335 per cent CD30\u2010positive cells). By PCR, 40 of 60 CD30\u2010positive NHLs (67 per cent) were EBV\u2010positive; in CD30\u2010negative cases, 6\/29 (21 per cent) were EBV\u2010positive, as were 12\/50 (24 per cent) reactive lymph nodes. The DISH procedure demonstrated the EBV genome exclusively in the nuclei of tumour cells in 23 of the 37 PCR EBV\u2010positive cases that were tested. PCR\u2010negative cases were always DISH\u2010negative, as were the PCR\u2010positive reactive lymph nodes and CD30\u2010negative NHLs. Immunohistochemistry demonstrated LMP in neoplastic cells of 7\/47 (15 per cent) CD30\u2010positive NHLs, both ALCL and non\u2010ALCL always in PCR EBV\u2010positive cases, but never in the two control groups. EBNA\u20102 staining could not be detected. It is concluded that EBV is present (and transcriptionally active) in a sizeable number of NHLs and an association between the presence of the EBV genome and CD30 expression seems likely.\n                  <\/jats:p>","DOI":"10.1002\/path.1711680311","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T12:12:11Z","timestamp":1119442331000},"page":"307-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["High incidence of EBV genome in CD30\u2010positive non\u2010Hodgkin's lymphomas"],"prefix":"10.1002","volume":"168","author":[{"given":"P.","family":"Kanavaros","sequence":"first","affiliation":[]},{"given":"N. M.","family":"Jiwa","sequence":"additional","affiliation":[]},{"given":"P. C.","family":"De Bruin","sequence":"additional","affiliation":[]},{"given":"P. Van Der","family":"Valk","sequence":"additional","affiliation":[]},{"given":"L. 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